進口SE型極化熒光光度計

High Sensitivity - Picomolar

Quantitative results

Minimal sample volumes and preparation

Rapid measurments

Programable Data collection

Variable Temperature Sample chamber

Easy to change external filter holders

Standard System 360-700nm

Expanded System 250-850nm

The Fluorescence Polarization Systems provide a rapid and precise way to measure equilibrium binding and/or molecular degradation, with picomolar sensitivity, for a wide range of biological molecules. Fluorescence polarization takes advantage of the change in the speed of rotation that occurs when a fluorescent molecule binds to another molecule. The Systems are useful in performing immunoassays, determining antibody specificity and affinity, and providing protein-DNA and receptor-ligand binding measurements. Affinity binding is measured directly in solution, with each data point obtained in just a few seconds (6 seconds minimum). This simple, direct approach eliminates the need for any filtration, gel mobility shift assays, or immobilization techniques, with fluorescent labeling eliminating the use of radioisotopes

The Fluorescence Polarization Systems are stand alone, bench-top instruments that offer sensitive polarization measurement (requiring as little as 10fmol/mL of fluorescently labled sample), in a single-tube-format. The instruments use standard disposable glass cuvettes and their small footprint

maximizes precious bench space. The instruments have an onboard operating software that lets the user store up to 99 user-defined protocols and send data directly to a printer or your personal computer for analysis.

Fluorescence Polarization System features:

Expanded System - 250-850nm combination Quartz Halogen and Mercury Vapor Lamps

Polarizers - Glan-Taylor prisms with Extinction coefficient >10,000 from 280-900nm

Standard System - 360-700nm Quartz Halogen lamp

Polarizers - Film type with Extinction coefficient >10,000 from 400-700nm

Both Instruments feature:

Filters - Special fluorescein Interference filters (490nm Excit. and 525nm Emiss.)

Easy filter exchange for use with wide range of fluophores

Sample format - Single tube, Cell holders for 6mm (100µl) and 10mm (500µl)

(5mm (25µl) is optional

Precision - <2mP S.D. at 1nM fluorescein for 0.5mL sample

Sensitivity - Accurate to 10-11M fluorescein in polarization mode

and 10-13M fluorescein in intensity mode

Temperature Control - Variable from 5°C to 75°C in 1°C increments,±1°C

Read Time - Minimum 6 seconds, variable number of read cycles

Programmable Protocols - 99 user defined, password protected

Interfaces - RS232-C, DB25 parallel

Printer - External Thermal (parallel) 40 column

LxH - 20.5” x 14.75” x 9.25” (52.6x37.8x23.7cm)

Net Weight - 35lbs. (15.9kg)