5通道96孔定量PCR儀
- 公司名稱 北京力途科技有限公司
- 品牌
- 型號
- 產(chǎn)地 韓國BIONEER
- 廠商性質 代理商
- 更新時間 2017/7/24 6:25:58
- 訪問次數(shù) 1474
聯(lián)系我們時請說明是化工儀器網(wǎng)上看到的信息,謝謝!
產(chǎn)品詳細描述
儀器簡介
Bioneer 運用分子生物學產(chǎn)品研發(fā)的豐富經(jīng)驗,結合的科技成果,研制出的五通道Exicycler™實時定量PCR儀。該產(chǎn)品在2004年被韓國產(chǎn)業(yè)資源部評為同類*質量產(chǎn)品之一。儀器由熱循環(huán)反應器和Bioneer*的實時熒光信號檢測模塊組成,利用熒光共振能量轉移原理,可以檢測兩種分子間的相互作用。具有高靈敏度、高準確性和系統(tǒng)實時整合等特性,該機器性能穩(wěn)定,帶梯度功能,試劑開放。擁有CE體外診斷認證,KFDA 醫(yī)療器械注冊證。
儀器特點
● 五通道,帶五個激發(fā)濾光片,五個檢測濾光片
● 光路技術,使用平面光源保證均勻的照明,孔間光照強度差異zui小化,無需 ROX 染料校正
● 16 bit 高靈敏度 2D CCD 檢測,可一次性獲得 96 孔中全部熒光信號,孔間無時間差
● 帶梯度計算和溫度梯度功能,便于PCR 反應條件優(yōu)化
● Touch down PCR 技術(包括Time increment、Temperature increment功能)
● 波長范圍 ( 490-670 nm ),支持常用的所有熒光染料和探針
● 動力學線性范圍10個數(shù)量級 (101 - 1010)
● 系統(tǒng)軟件界面友好,直觀,易于學習和操作
● 試劑開放,耗材經(jīng)濟
● 認證——CE-IVD,KFDA醫(yī)療器械注冊證
儀器優(yōu)勢
出色的2 - Dimensinal激發(fā)光源
● 采用的光路技術,使用平面光源保證均勻的照明,無邊緣效應
● 孔間光照強度差異zui小化
● 無需使用內參染料校正
增強的光學模塊和檢測系統(tǒng)
● 使用高靈敏度2 D CCD檢測,可一次性獲得96孔中全部熒光信號
● 孔間無時間差
● 超高壓短弧光燈的明亮白光為所有的顏色提供均一的光照強度
● 光路模塊可將所有孔內光損失降至zui低
● 更長的燈泡使用壽命
可靠的Ct曲線
● 在沒有使用內參染料校正的條件下,重復的樣本間可以獲得*質量的Ct曲線
五通道技術
● 無需為內參染料保留一個通道,所有的五色通道都是可用的。
應用范圍
● 基因表達定量
● 病原體檢測
● 微陣列分析
● 細菌計數(shù)/病毒滴度以及鑒定反應產(chǎn)物
● 基于解鏈溫度的基因型檢測
實驗結果
*的光學系統(tǒng)允許樣品的濃度信號超出系統(tǒng)的動態(tài)、靈敏、線形的檢測范圍。
圖1.使用Accupower® GreenStar™ PCR預混合物(Bioneer貨號:K-5040)獲得的線性動態(tài)曲線和靈敏定量結果。(A和B)將目的DNA樣品10倍系列稀釋后,分別擴增出100 bp的產(chǎn)物。(C) C(T)值介于3.2-3.4,理論值為3.3。(D)標準曲線結果顯示相關系數(shù)超過0.999。
圖2.重復性。在不同的孔中擴增出150 bp的相同目的DNA片段,設置8個平行實驗。
極易操控、靈活直觀的操作軟件
Exicycler™操作系統(tǒng)以圖形界面(GUI)為基礎,極易學習、掌握、操作,且功能強大。并行的結果分析功能支持在實驗進行中同時進行獨立的操作和數(shù)據(jù)分析。該軟件系統(tǒng)提供持續(xù)升級,能適應不同的操作和特征分析。
圖3.Exicycler™軟件的使用
Exicycler™實時定量PCR儀技術參數(shù)
系統(tǒng)參數(shù) | |
尺寸(mm) | 540(長) * 355(寬) * 470(高) |
重量 | 30 kg |
樣本容量 | 96 孔 / 0.2 ml管 |
樣本體積 | 20 ~ 50 μl ( 50 μl recommended ) |
電源指標 | 110 V/240 V, 50/60 Hz, 850 W |
環(huán)境溫度 | 15℃-30℃ |
環(huán)境濕度 | 20-80 |
熱學模塊參數(shù) | |
溫控方式 | 半導體( Peltier ) |
溫度范圍 | 4℃ - 99.9℃ |
溫度精度 | ±0.3℃ |
變溫速率 | 2.5℃/sec |
梯度功能 | 1℃-20℃ within 40℃-75℃ |
溫度/時間增量范圍 | 0.1℃-9.9℃ / 1sec-60 sec |
光學模塊參數(shù) | |
光源, | 超高壓短弧光燈( 120 W ) |
燈泡時長 | 3,000 小時 |
探測器 | 16 位 2D CCD |
激發(fā)光濾片/檢測光濾片 | 各5個 |
計算機參數(shù) | |
操作系統(tǒng) | Windows XP |
處理速度 | In Dual Core E2160 (1.8 GHz ) 或更高 |
內存 | 1 GB 或更高 |
數(shù)據(jù)接口 | USB 2.0 或更高 |
顯示器分辨率 | 1280*1024或更高 |
證書
使用Exicycler™ 96發(fā)表的文章
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