Leica TCS SP8 激光掃描共聚焦顯微鏡
- 公司名稱 廣州市炳陽(yáng)生物科技有限公司
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- 更快的成像速度支持新的生物學(xué)應(yīng)用
- 隨時(shí)根據(jù)需要成長(zhǎng)的共聚焦系統(tǒng)
Leica TCS SP8 – 創(chuàng)新點(diǎn)概覽
STED Key Publications
First publication on STED technology:
Hell, S. W. & Wichmann, J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics letters 19, 780-782, (1994).http://www.ncbi.nlm.nih.gov/pubmed/19844443
First biological image:
Klar, T. A., Jakobs, S., Dyba, M., Egner, A. & Hell, S. W. Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proceedings of the National Academy of Sciences of the United States of America 97, 8206-8210, (2000). http://www.ncbi.nlm.nih.gov/pubmed/10899992
STED with genetically encoded marker: GFP
Willig, K. I. et al. Nanoscale resolution in GFP-based microscopy. Nature methods 3, 721-723, (2006).http://www.ncbi.nlm.nih.gov/pubmed/16896340
STED with continuous wave laser beams:
Willig, K. I., Harke, B., Medda, R. & Hell, S. W. STED microscopy with continuous wave beams. Nature methods 4, 915-918, (2007). http://www.ncbi.nlm.nih.gov/pubmed/17952088
Two-color STED with two STED lines
Donnert, G. et al. Two-color far-field fluorescence nanoscopy. Biophysical journal 92, L67-69, (2007).http://www.ncbi.nlm.nih.gov/pubmed/17307826
Iso-STED and two-color STED with one STED line
Schmidt, R. et al. Spherical nanosized focal spot unravels the interior of cells. Nature methods 5, 539-544, (2008).http://www.ncbi.nlm.nih.gov/pubmed/18488034
STED-FCS application
Eggeling, C. et al. Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature 457, 1159-1162, (2009). http://www.ncbi.nlm.nih.gov/pubmed/19098897
Gated STED
Vicidomini, G. et al. Sharper low-power STED nanoscopy by time gating. Nature methods 8, 571-573, (2011).http://www.ncbi.nlm.nih.gov/pubmed/21642963
Selected Publications with Leica STED instrument
2016
Qiu, Z. et al. Translation Microscopy (TRAM) for super-resolution imaging. Scientific reports 6, 19993, (2016).http://www.ncbi.nlm.nih.gov/pubmed/26822455
Ruge, C. A. et al. Disintegration of nano-embedded microparticles after deposition on mucus: A mechanistic study. Colloids and surfaces. B, Biointerfaces 139, 219-227, (2016). http://www.ncbi.nlm.nih.gov/pubmed/26720142
Scheuring, D. et al. Actin-dependent vacuolar occupancy of the cell determines auxin-induced growth repression. Proceedings of the National Academy of Sciences of the United States of America 113, 452-457, (2016).http://www.ncbi.nlm.nih.gov/pubmed/26715743
Torreno-Pina, J. A. et al. The actin cytoskeleton modulates the activation of iNKT cells by segregating CD1d nanoclusters on antigen-presenting cells. Proceedings of the National Academy of Sciences of the United States of America 113, E772-781, (2016). http://www.ncbi.nlm.nih.gov/pubmed/26798067
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