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化工儀器網(wǎng)>產(chǎn)品展廳>生命科學(xué)儀器>組織學(xué)設(shè)備>其它組織學(xué)設(shè)備>EpiAirway 3D human tissue EpiAirway 3D體外呼吸組織模型,mattek代理

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EpiAirway 3D human tissue EpiAirway 3D體外呼吸組織模型,mattek代理

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世聯(lián)博研(北京)科技有限公司(Bio Excellence International Tech Co.,Ltd)簡稱為世聯(lián)博研。世聯(lián)博研是一家集進(jìn)口科研儀器代理銷售以及實驗技術(shù)服務(wù)于一體的技術(shù)公司。世聯(lián)博研專注生物力學(xué)和3D生物打印前沿科研設(shè)備代理銷售及科研實驗項目合作服務(wù),內(nèi)容涵蓋了血管力學(xué)生物學(xué)、生物力學(xué)建模仿真與應(yīng)用、細(xì)胞分子生物力學(xué)、組織修復(fù)生物力學(xué)、骨與關(guān)節(jié)生物力學(xué)、口腔力學(xué)生物學(xué)、眼耳鼻咽喉生物力學(xué)、康復(fù)工程生物力學(xué)、生物材料力學(xué)與仿生學(xué)、人體運(yùn)動生物力學(xué)等生物力學(xué)研究以及生物材料打印、打印樣品生物力學(xué)性能測試分析的前沿領(lǐng)域科研利器和科研服務(wù)。

世聯(lián)博研的客戶范圍:
科研院所單位、生物醫(yī)學(xué)科研高校、醫(yī)院基礎(chǔ)科研單位等。

世聯(lián)博研公司代理的品牌具有:
1)近10年長期穩(wěn)定的貨源
2)以生物力學(xué)、細(xì)胞力學(xué)、細(xì)胞生物分子學(xué)、生物醫(yī)學(xué)組織工程、生物材料學(xué)為主,兼顧其他相關(guān)產(chǎn)品線
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4)良好的技術(shù)支持
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6)每年新增的貨源。

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EpiAirway 3D體外呼吸組織模型,mattek代理,EpiAirway 3D human tissue

 

 

 

EpiAirway 3D體外呼吸組織模型

EpiAirway™-MatTek的人類3D體外呼吸組織模型正在重新定義毒理學(xué),病毒研究,炎癥和纖維化以及藥物開發(fā)領(lǐng)域的臨床前測試。

EpiAirway™
MatTek的人類氣道3D組織模型正在范圍內(nèi)推進(jìn)體外呼吸研究。 EpiAirway允許與病原體,化學(xué)物質(zhì)或治療劑發(fā)生生理接觸,因此與人類有關(guān)的生物學(xué)反應(yīng)正在改變科學(xué)家研究呼吸系統(tǒng)疾病和藥物開發(fā)的方式。 讓EpiAirway推動您的科學(xué)發(fā)展。
粘液纖毛分化
由健康或患病的原代人類細(xì)胞產(chǎn)生
提供30多個捐助者
交付即用

 

 

EpiAirway Histology New

EpiAirwayFT Histology New

EpiAirway is a ready-to-use, 3D mucociliary tissue model consisting of normal, human-derived tracheal/bronchial epithelial cells also available as a co-culture system with normal human stromal fibroblasts (EpiAirwayFT). Cultured at the air-liquid interface (ALI), EpiAirway recapitulates the in vivo phenotypes of barrier, mucociliary responses, infection, toxicity responses and disease. With over 100 technical references available, EpiAirway is amenable to acute or long-term chronic studies across a wide range of highly predictive in vitro applications.

EpiAirway 6-week CultureEpiAirway at 6 weeks in culture.

EpiAirway exhibits human relevant tissue structure and cellular morphology with high uniformity and reproducibility. Its 3D structure consists of organized Keratin 5+ basal cells, mucus producing goblet cells, functional tight junctions and beating cilia. EpiAirwayFT incorporates human fibroblasts in an extracellular stromal matrix ideal for inflammation and fibrosis research.

A standard EpiAirway kit (AIR-100) consists of 24 tissues. (Tissue “kits” contain tissues, a small amount of culture medium, and plasticware; contact MatTek for specific kit contents)

Formats:

 

  • 9mm & 22mm individual inserts – tissue culture substrate is chemically modified with a pore size of 0.4 μm
  • 24mm, 12mm & 6.5mm Transwell inserts with a pore size of 0.4 μm
  • 96-well HTS plates – tissue culture substrate is chemically modified with a pore size of 0.4 μm

Culture: Air-liquid interface

Histology: 3-4 cell layers – pseudostratified, mucociliary morphology and tissue structure

Lot numbers: Tissue lots produced each week are assigned a specific lot number. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. All tissue kits within a lot are identical in regards to cells, medium, handling, culture conditions, etc.

Shipment: At 4°C on medium-supplemented, agarose gels

Shipment day: Every Monday

Delivery: Tuesday morning via FedEx priority service (US). Outside US: Tuesday-Thursday depending on location

Shelf life: Including time in transit, tissues may be stored at 4°C for up to 3 days prior to use. However, extended storage periods are not recommended unless necessary. In addition, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning)

Length of experiments: Tissue cultures can be continued for THREE (3) MONTHS or more with good retention of normal morphology. Tissues must be fed every other day with 5.0 ml of maintenance medium (AIR-100-MM).  Cell culture inserts are placed atop washers (EPI-WSHR) or culture stands (MEL-STND) in 6-well plates to allow us of 5.0 ml.  See technical reference #631.

 

Cells

Type: Normal human tracheal/bronchial epithelial cells (NHBE);

Genetic make-up: Single donor

Derived from: Healthy, non-smoker (alternate donors available upon request)

Alternatives: NHBE from Asthmatic, COPD and Smoker donors

Screened for: HIV, Hepatitis-B, Hepatitis-C, mycoplasma

 

Medium

Base medium: Dulbecco’s Modified Eagle’s Medium (DMEM)

Growth factors/hormones: Epidermal growth factors and other proprietary factors

Serum: None

Antibiotics: Gentamicin 5 µg/ml (10% of normal gentamicin level)

Anti-fungal agent: Amphotericin B 0.25 µg/ml

pH Indicator: Phenol red

Other additives: Proprietary

Alternatives: Phenol red-free, antibiotic-free, anti-fungal-free medium and tissues are available. Agents are removed at least 3 days prior to shipment.

Assay medium: AIR-100-ASY

Maintenance medium: AIR-100-MM

 

Quality Control and Sterility

Visual inspection: All tissues are visually inspected and if physical imperfections are noted, tissues are rejected for shipment

End-use testing: Transepithelial electrical resistance (TEER) of each EpiAirway lot is measured using an EVOM2 epithelial Volthommeter and Endohm electrode chamber (World Precision Instruments). A minimum TEER of 300 Ohm • cm2 is required for QC release.

Sterility: All media used throughout the production process is checked for sterility. Maintenance medium is incubated without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination

Screening for pathogens: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen free. Thus, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, “Biosafety in microbiological and biomedical laboratories,” 1998. For further assistance, please contact your site Safety Officer or MatTek technical service

Notification of lot failure: If a tissue lot fails our QC or sterility testing, the customer will be notified and the tissues will be replaced without charge when appropriate. Because our QC and sterility testing is done post-shipment, notification will be made as soon as possible (Under normal circumstances, TEER failures will be notified by Wednesday 5 p.m.; sterility failures will be notified within 8 days of shipment)

 

 

應(yīng)用:

 

The EpiAirway 3D human tissue model is routinely utilized for a range of applications including safety & risk assessment, anti-viral research, inflammation & fibrosis and drug delivery. Simple protocols and the evaluation of early cellular endpoints allow scientists to acquire data in days, not weeks or months.

Virus Infection

Use EpiAirway to study respiratory virus attachment, replication, innate immune responses and anti-viral drug development.

Viral Infection Reference

Drug Delivery

Utilize existing transdermal permeation equipment or MatTek’s simple single insert permeation devices to assess API permeation and flux.

Drug Delivery Protocol

Drug Delivery Reference

Inhalation Toxicology

Use EpiAirway (available in several formats for physiological exposure systems) to determine relative safety with the EpiAirway IC-75 assay.

EpiAirway Toxicity Protocol

Inhalation Toxicology Reference

Inflammation and Fibrosis

Employ EpiAirway’s diverse donor inventory of healthy and diseased donors (Asthma, COPD, Smoker, Goblet Cell Hyperplasia) to elucidate molecular pathways and assess therapeutic efficacy.

View the MatTek Reference Library

 

 

 

EpiAirway 3D體外呼吸組織模型

EpiAirway™-MatTek的人類3D體外呼吸組織模型正在重新定義毒理學(xué),病毒研究,炎癥和纖維化以及藥物開發(fā)領(lǐng)域的臨床前測試。

EpiAirway™
MatTek的人類氣道3D組織模型正在范圍內(nèi)推進(jìn)體外呼吸研究。 EpiAirway允許與病原體,化學(xué)物質(zhì)或治療劑發(fā)生生理接觸,因此與人類有關(guān)的生物學(xué)反應(yīng)正在改變科學(xué)家研究呼吸系統(tǒng)疾病和藥物開發(fā)的方式。 讓EpiAirway推動您的科學(xué)發(fā)展。
粘液纖毛分化
由健康或患病的原代人類細(xì)胞產(chǎn)生
提供30多個捐助者
交付即用

 

 

EpiAirway Histology New

EpiAirwayFT Histology New

EpiAirway is a ready-to-use, 3D mucociliary tissue model consisting of normal, human-derived tracheal/bronchial epithelial cells also available as a co-culture system with normal human stromal fibroblasts (EpiAirwayFT). Cultured at the air-liquid interface (ALI), EpiAirway recapitulates the in vivo phenotypes of barrier, mucociliary responses, infection, toxicity responses and disease. With over 100 technical references available, EpiAirway is amenable to acute or long-term chronic studies across a wide range of highly predictive in vitro applications.

EpiAirway 6-week CultureEpiAirway at 6 weeks in culture.

EpiAirway exhibits human relevant tissue structure and cellular morphology with high uniformity and reproducibility. Its 3D structure consists of organized Keratin 5+ basal cells, mucus producing goblet cells, functional tight junctions and beating cilia. EpiAirwayFT incorporates human fibroblasts in an extracellular stromal matrix ideal for inflammation and fibrosis research.

A standard EpiAirway kit (AIR-100) consists of 24 tissues. (Tissue “kits” contain tissues, a small amount of culture medium, and plasticware; contact MatTek for specific kit contents)

Formats:

 

  • 9mm & 22mm individual inserts – tissue culture substrate is chemically modified with a pore size of 0.4 μm
  • 24mm, 12mm & 6.5mm Transwell inserts with a pore size of 0.4 μm
  • 96-well HTS plates – tissue culture substrate is chemically modified with a pore size of 0.4 μm

Culture: Air-liquid interface

Histology: 3-4 cell layers – pseudostratified, mucociliary morphology and tissue structure

Lot numbers: Tissue lots produced each week are assigned a specific lot number. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. All tissue kits within a lot are identical in regards to cells, medium, handling, culture conditions, etc.

Shipment: At 4°C on medium-supplemented, agarose gels

Shipment day: Every Monday

Delivery: Tuesday morning via FedEx priority service (US). Outside US: Tuesday-Thursday depending on location

Shelf life: Including time in transit, tissues may be stored at 4°C for up to 3 days prior to use. However, extended storage periods are not recommended unless necessary. In addition, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning)

Length of experiments: Tissue cultures can be continued for THREE (3) MONTHS or more with good retention of normal morphology. Tissues must be fed every other day with 5.0 ml of maintenance medium (AIR-100-MM).  Cell culture inserts are placed atop washers (EPI-WSHR) or culture stands (MEL-STND) in 6-well plates to allow us of 5.0 ml.  See technical reference #631.

 

Cells

Type: Normal human tracheal/bronchial epithelial cells (NHBE);

Genetic make-up: Single donor

Derived from: Healthy, non-smoker (alternate donors available upon request)

Alternatives: NHBE from Asthmatic, COPD and Smoker donors

Screened for: HIV, Hepatitis-B, Hepatitis-C, mycoplasma

 

Medium

Base medium: Dulbecco’s Modified Eagle’s Medium (DMEM)

Growth factors/hormones: Epidermal growth factors and other proprietary factors

Serum: None

Antibiotics: Gentamicin 5 µg/ml (10% of normal gentamicin level)

Anti-fungal agent: Amphotericin B 0.25 µg/ml

pH Indicator: Phenol red

Other additives: Proprietary

Alternatives: Phenol red-free, antibiotic-free, anti-fungal-free medium and tissues are available. Agents are removed at least 3 days prior to shipment.

Assay medium: AIR-100-ASY

Maintenance medium: AIR-100-MM

 

Quality Control and Sterility

Visual inspection: All tissues are visually inspected and if physical imperfections are noted, tissues are rejected for shipment

End-use testing: Transepithelial electrical resistance (TEER) of each EpiAirway lot is measured using an EVOM2 epithelial Volthommeter and Endohm electrode chamber (World Precision Instruments). A minimum TEER of 300 Ohm • cm2 is required for QC release.

Sterility: All media used throughout the production process is checked for sterility. Maintenance medium is incubated without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination

Screening for pathogens: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. However, no known test method can offer complete assurance that the cells are pathogen free. Thus, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, “Biosafety in microbiological and biomedical laboratories,” 1998. For further assistance, please contact your site Safety Officer or MatTek technical service

Notification of lot failure: If a tissue lot fails our QC or sterility testing, the customer will be notified and the tissues will be replaced without charge when appropriate. Because our QC and sterility testing is done post-shipment, notification will be made as soon as possible (Under normal circumstances, TEER failures will be notified by Wednesday 5 p.m.; sterility failures will be notified within 8 days of shipment)

 

 

應(yīng)用:

 

The EpiAirway 3D human tissue model is routinely utilized for a range of applications including safety & risk assessment, anti-viral research, inflammation & fibrosis and drug delivery. Simple protocols and the evaluation of early cellular endpoints allow scientists to acquire data in days, not weeks or months.

Virus Infection

Use EpiAirway to study respiratory virus attachment, replication, innate immune responses and anti-viral drug development.

Viral Infection Reference

Drug Delivery

Utilize existing transdermal permeation equipment or MatTek’s simple single insert permeation devices to assess API permeation and flux.

Drug Delivery Protocol

Drug Delivery Reference

Inhalation Toxicology

Use EpiAirway (available in several formats for physiological exposure systems) to determine relative safety with the EpiAirway IC-75 assay.

EpiAirway Toxicity Protocol

Inhalation Toxicology Reference

Inflammation and Fibrosis

Employ EpiAirway’s diverse donor inventory of healthy and diseased donors (Asthma, COPD, Smoker, Goblet Cell Hyperplasia) to elucidate molecular pathways and assess therapeutic efficacy.

View the MatTek Reference Library

 

 



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