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化工儀器網(wǎng)>產(chǎn)品展廳>生命科學儀器>細胞培養(yǎng)儀器>細胞培養(yǎng)系統(tǒng)>flexcell str-4000 flexcell細胞剪切力學實驗裝置系統(tǒng)

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flexcell str-4000 flexcell細胞剪切力學實驗裝置系統(tǒng)

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世聯(lián)博研(北京)科技有限公司(Bio Excellence International Tech Co.,Ltd)簡稱為世聯(lián)博研。世聯(lián)博研是一家集進口科研儀器代理銷售以及實驗技術(shù)服務(wù)于一體的技術(shù)公司。世聯(lián)博研專注生物力學和3D生物打印前沿科研設(shè)備代理銷售及科研實驗項目合作服務(wù),內(nèi)容涵蓋了血管力學生物學、生物力學建模仿真與應(yīng)用、細胞分子生物力學、組織修復生物力學、骨與關(guān)節(jié)生物力學、口腔力學生物學、眼耳鼻咽喉生物力學、康復工程生物力學、生物材料力學與仿生學、人體運動生物力學等生物力學研究以及生物材料打印、打印樣品生物力學性能測試分析的前沿領(lǐng)域科研利器和科研服務(wù)。

世聯(lián)博研的客戶范圍:
科研院所單位、生物醫(yī)學科研高校、醫(yī)院基礎(chǔ)科研單位等。

世聯(lián)博研公司代理的品牌具有:
1)近10年長期穩(wěn)定的貨源
2)以生物力學、細胞力學、細胞生物分子學、生物醫(yī)學組織工程、生物材料學為主,兼顧其他相關(guān)產(chǎn)品線
3)提供專業(yè)產(chǎn)品培訓和銷售培訓
4)良好的技術(shù)支持
5)已成交老客戶考證
6)每年新增的貨源。

細胞應(yīng)力加載儀,3細胞打印機,NanoTweezer新型激光光鑷系統(tǒng),PicoTwist磁鑷,美國NeuroIndx品牌Kuiqpick單細胞捕獲切割系統(tǒng)

應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè)

細胞剪切力學實驗系統(tǒng),flexcell str-4000

美國flexcell品牌,型號:str-4000

細胞剪切力學實驗系統(tǒng),可提供穩(wěn)流式切應(yīng)力、脈沖式切應(yīng)力或者往返式切應(yīng)力多種流場刺激

細胞剪切力學實驗系,flexcell str-4000,細胞切應(yīng)力實驗系統(tǒng),藥代動力學實驗裝置,可提供穩(wěn)流式切應(yīng)力、脈沖式切應(yīng)力或者往返式切應(yīng)力多種流場刺激,在實現(xiàn)細胞在流體切應(yīng)力的同時抻拉,實時觀察細胞在多力混合加載刺激下的變化反應(yīng),細胞量大,便于后期分析,便于后期分析細胞中基因的表達(mRNA檢測),以及使用流式細胞儀、基因芯片等進行分析,產(chǎn)品成熟度與使用案例:國外內(nèi)有數(shù)千篇文獻

Flexflow單通道平行板流室系統(tǒng)提供流體切應(yīng)力同時抻拉細胞


FlexcellFlexFlow顯微切應(yīng)力加載設(shè)備(SHEAR Stress device)

  • 可以在提供流體切應(yīng)力的同時抻拉細胞,測試血管和結(jié)綈組織細胞對液體流動的實時反應(yīng)。
  • 為培育在StageFlexer硅膠模表面或者基質(zhì)蛋白包被的細胞培養(yǎng)片上的細胞提供切應(yīng)力。
  • 使用FX-5000T應(yīng)力加載系統(tǒng)抻拉細胞,并且可以在實驗前,實驗中或者實驗后提供切應(yīng)力。
  • 計算機控制蠕動泵,調(diào)節(jié)切應(yīng)力大小,從0-35 dynes/cm2
  • 使用標準正立式顯微鏡實時觀察細胞在切應(yīng)力下的反應(yīng)。
  • 檢測細胞在流體作用下的排列反應(yīng)。
  • 加力同時實時檢測在液體切應(yīng)力下各種激活劑/抑制劑對細胞反應(yīng)的影響。使用熒光團例如FURA-2檢測細胞內(nèi)[Ca2+]ic或者其它離子對切應(yīng)力反應(yīng)

典型應(yīng)用文獻:

1. Archambault JM, Elfervig MK, Tsuzaki M, Herzog W, Banes AJ. Shear stress response of rabbit tendon cells is serum dependent. Proceedings of the Eleventh Canadian Society for Biomechanics Conference, 181, 2000.
2. Archambault JM, Elfervig-Wall MK, Tsuzaki M, Herzog W, Banes AJ. Rabbit tendon cells produce MMP-3 in response to fluid flow without significant calcium transients. J Biomech 35(3):303-309, 2002.

3. Clark PR, Jensen TJ, Kluger MS, Morelock M, Hanidu A, Qi Z, Tatake RJ, Pober JS. MEK5 is activated by shear stress, activates ERK5 and induces KLF4 to modulate TNF responses in human dermal microvascular endothelial cells. Microcirculation 18(2):102-117, 2011.
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5. Eifler RL, Blough ER, Dehlin JM, Haut Donahue TL. Oscillatory fluid flow regulates glycosaminoglycan production via an intracellular calcium pathway in meniscal cells. J Orthop Res 24(3):375-384, 2006.
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7. Elfervig M, Lotano M, Tsuzaki M, Faber J, Banes A J. Fluid-induced shear stress modulates Cx-43 expression in avian tendon cells but does not induce a Ca2+ signal [abstract]. Transactions of the 47th Annual Meeting of the Orthopaedic Research Society 26:570, 2001.
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20. Jaitovich A, Mehta S, Na N, Ciechanover A, Goldman RD, Ridge KM. Ubiquitin-proteasome-mediated degradation of keratin intermediate filaments in mechanically stimulated A549 cells. J Biol Chem 283(37):25348-25355, 2008.
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23. Li M, Liu X, Zhang Y, Di M, Wang H, Wang L, Chen Y, Liu X, Cao X, Zeng R, Zhang Y, Zhang M. Upregulation of Dickkopf1 by oscillatory shear stress accelerates atherogenesis. J Mol Med (Berl) 94(4):431-41, 2016.
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26. Malone AM, Batra NN, Shivaram G, Kwon RY, You L, Kim CH, Rodriguez J, Jair K, Jacobs CR. The role of actin cytoskeleton in oscillatory fluid flow-induced signaling in MC3T3-E1 osteoblasts. Am J Physiol Cell Physiol 292(5):C1830-C1836, 2007.
27. Maycas M, Ardura JA, de Castro LF, Bravo B, Gortázar AR, Esbrit P. Role of the parathyroid hormone type 1 receptor (PTH1R) as a mechanosensor in osteocyte survival. J Bone Miner Res 30(7):1231-44, 2015.
28. Maycas M, Bravo-Molina B, Fernández de Castro L, Pozuelo JM, Forriol F, P Esbrit, Rodríguez de Gortázar A. High glucose alters the antiapoptotic response to mechanical stimulation in MLO-Y4 osteocytic cells. Trauma Fund MAPFRE 25(2):97-100, 2014.
29. Metaxa E, Meng H, Kaluvala SR, Szymanski MP, Paluch RA, Kolega J. Nitric oxide-dependent stimulation of endothelial cell proliferation by sustained high flow. Am J Physiol Heart Circ Physiol 295(2):H736-H742, 2008.
30. Ni J, Waldman A, Khachigian LM. c-Jun regulates shear- and injury-inducible Egr-1 expression, vein graft stenosis after autologous end-to-side transplantation in rabbits, and intimal hyperplasia in human saphenous veins. J Biol Chem 285(6):4038-4048, 2010.
31. Qi J, Chi L, Faber J, Koller B, Banes AJ. ATP reduces gel compaction in osteoblast-populated collagen gels. J Appl Physiol 102(3):1152-60, 2007.
32. Radel C, Carlile-Klusacek M, Rizzo V. Participation of caveolae in ?1 integrin-mediated mechanotransduction. Biochem Biophys Res Commun 358(2):626-631, 2007.
33. Radel C, Rizzo V. Integrin mechanotransduction stimulates caveolin-1 phosphorylation and recruitment of Csk to mediate actin reorganization. Am J Physiol Heart Circ Physiol 288(2):H936-H945, 2005.
34. Ridge KM, Linz L, Flitney FW, Kuczmarski ER, Chou YH, Omary MB, Sznajder JI, Goldman RD. Keratin 8 phosphorylation by protein kinase C ? regulates shear stress-mediated disassembly of keratin intermediate filaments in alveolar epithelial cells. J Biol Chem 280(34):30400-30405, 2005.
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