化工儀器網(wǎng)>產(chǎn)品展廳>生命科學(xué)儀器>分子生物學(xué)儀器>蛋白/肽測(cè)序儀>Human Transferrin protein 重組人轉(zhuǎn)鐵蛋白（TF）
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| 產(chǎn)品英文名稱 | Human Transferrin protein |
| 產(chǎn)品中文名稱 | 重組人轉(zhuǎn)鐵蛋白（TF） |
| 別名 | PRO2086 Protein; TFQTL1 Protein; HEL-S-71p Protein, Human; PRO1557 Protein; Transferrin Protein,Siderophilin, partially saturated |
| 標(biāo)簽 | 無標(biāo)簽 |
| 種屬 | 人 |
| 序列 | Amino acid sequence derived from Human Transferrin protein isoform (P02787, Met 1-Pro 698) with a polyhistidine tag at the C-terminus was expressed. |
| 活性 | 檢測(cè)進(jìn)行中 |
| 蛋白長(zhǎng)度 | 重組人轉(zhuǎn)鐵蛋白由690個(gè)氨基酸組成，預(yù)測(cè)分子量為76.6 kDa。 在還原條件下，SDS-PAGE中人轉(zhuǎn)鐵蛋白的表觀分子量約為70-100 kDa。 |
| 純度 | ≥ 95 % ，使用SDS-PAGE檢測(cè) |
| 特點(diǎn)&優(yōu)勢(shì) | 采用 LAL 法檢測(cè)，每 1 μg 蛋白質(zhì)中的 EU 含量小于 1.0。 |
| 產(chǎn)品形式 | 凍干粉，凍干緩沖液為無菌的 PBS，pH 7.4。 |
| 基因ID | 7018 |
| 蛋白序列鏈接 | /uniprot/P02787 |
| 使用中注意事項(xiàng) | 開蓋使用前，請(qǐng)先離心。本產(chǎn)品為凍干粉，推薦用該產(chǎn)品配套的Reconstitution Buffer進(jìn)行復(fù)溶，且濃度不低于100 µg/mL?？蛻艨筛鶕?jù)后續(xù)的實(shí)驗(yàn)需求，進(jìn)行進(jìn)一步的稀釋。 |
| 保存建議 | 本產(chǎn)品為凍干粉形式，建議：將凍干粉的重組人轉(zhuǎn)鐵蛋白（TF）產(chǎn)品保存于-20℃以下的干燥環(huán)境中。本產(chǎn)品溶解之后，可在4℃保存2-7天，長(zhǎng)期使用請(qǐng)置于-20℃以下。為長(zhǎng)期保存，推薦添加載體蛋白（0.1% HSA或者BSA），并酌情分裝保存，避免反復(fù)凍融。 |
| 運(yùn)輸條件 | 常溫運(yùn)輸 |
| 警告 | 本文列出的產(chǎn)品僅供研究使用，不適用于人類或臨床診斷。我們產(chǎn)品所推薦應(yīng)用，不是建議使用我們的產(chǎn)品去違反任何或許可證。對(duì)于使用本產(chǎn)品可能發(fā)生的侵權(quán)或其他違規(guī)行為，我們不承擔(dān)任何責(zé)任。 |
| 背景 | Transferrin (Tf),is an iron transporter, which is mainly biosynthesized in the liver, and it can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc.The glycans of serum-type Tf in the CSF are similar to those of Tf in serum. Biochemical analyses reveal that the apparent molecular size of brain-type Tf is smaller than that of serum-type Tf, and that hydrophobic patches are exposed on brain-type Tf as demonstrated by hydrophobic probe binding studies. Research indicates that brain-type Tf levels is decreased in idiopathic normal pressure hydrocephalus, in which CSF production is suspected to decrease, while brain-type Tf increased in spontaneous intracranial hypotension, in which CSF production is suspected to increase. These results suggest that brain-type Tf could be a biomarker of altered CSF production. |
Fig.SDS-PAGE analysis of Human Transferrin protein.
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