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品名：G418 SULFATE

CAS Number: 108321-42-2

性狀：白色粉末

存儲溫度：2 to 6°C

分子式: C₂₀H₄₀N₄O₁₀·2H₂SO₄

分子量: 692.7

溶劑：溶于水

用途：G418是一種氨基糖苷類抗生素，通過干擾核糖體的功能而阻斷細胞的蛋白合成。在基因轉(zhuǎn)移、基因敲除、抗性篩選以及轉(zhuǎn)基因動物等方面得以廣泛應(yīng)用，是穩(wěn)定轉(zhuǎn)染zui常用的抗性篩選試劑。

產(chǎn)品信息

G418 is an aminoglycoside antibiotic. Unlike other aminoglycoside, G418 is more potent because it contains a hydroxyl function at C-6’ position rather than an amino function. It binds directly to the 80S ribosomal subunit and inhibits protein synthesis of eukaryotic cells.1 G418 is effective against aerobics and facultative Gram-negative bacilli, and some Gram-positive bacteria.

In molecular biology application, G418 sulfate is often used as a selection agent

Please Note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.

References

1. Marie-Paule Mingeot-Leclercq, Youri Glupczynski, and Paul M. Tulkens. 1999. Aminoglycosides: Activity and Resistance. Antimicrob Agents Chemother. Vol 43(4). Pp. 727-737.

2. Xie, D. and Y. Hong. 2002. Agrobacterium-mediated genetic transformation of Acacia mangium. Plant Cell Reports. Vol 20(10). Pp. 917-922.

3. P. A. Lazzeri, R. Brettschneider, R. Lührs, and H. Lörz. 1991. Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts. Theoretical and Applied Genetics. Vol 81(4). Pp. 437-444.

4. Christine Lang-Hinrichs, Dietmar Berndorff, Carsten Seefeldt, and Ulf Stahl. 1989. G418 resistance in the yeast Saccharomyces cerevisiae: comparison of the neomycin resistance genes from Tn5 and Tn903. Applied Microbiology and Biotechnology. Vol 30(4). Pp. 388-394.

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