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CF488A是一種針對488nm氬離子激光器優(yōu)化的綠色熒光染料。在通常的檢測條件下，CF488A的亮度至少和Alexa Fluor 488 相當。但CF488A相對于Alexa Fluor 488有一個明顯的優(yōu)勢，用CF488A制備的熒光偶聯(lián)抗體的生物學(xué)特異性更好。Alexa Fluor 488本身帶有多個負電荷，將會顯著的改變被標記蛋白的等電點，因此也就會改變用它標記的熒光偶聯(lián)抗體的生物學(xué)特異性。與此相反，CF488A幾乎不帶電荷，用它制備的熒光偶聯(lián)抗體可以保證檢測的特異性并提高信噪比。CF488A的另一個優(yōu)點是它的峰值發(fā)射波長比Alexa Fluor 488短10nm，比傳統(tǒng)的FITC(FAM)染料短15nm。因此，在多波長檢測的時候，CF488A可以顯著減少在紅色通道的溢出效應(yīng)，從而減小對紅色熒光的干擾。
表A-C 為CF488A產(chǎn)品目錄，我們提供全套的CF488A熒光偶聯(lián)二抗，抗體標記試劑盒，以及其他生物分子偶聯(lián)CF488A的產(chǎn)品，包括：鬼筆環(huán)肽，膜聯(lián)蛋白 V和a-銀環(huán)蛇毒素等偶聯(lián)產(chǎn)品。
南京賽泓瑞生物為Biotium公司 的一級代理商，： :
技術(shù)資料：
優(yōu)勢：
| 圖1.CF488A（藍色）， Alexa Fluor 488（綠色）和FITC（紅色）偶聯(lián)羊抗鼠IgG 二抗在PBS溶液中的激發(fā)和發(fā)射光譜。 |
圖2. 用胞外CD3抗體或同型對照抗體（BD Biosciences）分別標記Jurkat細胞，然后分別用偶聯(lián)圖中所示廠商熒光基團的羊抗鼠IgG二抗染色。插圖顯示的是用Beckman Coulter FC-500檢測到的細胞計數(shù)的中位數(shù)熒光值。直方圖顯示的是非別用CF488A（綠色），AlexaFluor488（藍色），DyLight488 Pierce（紫色）和DyLight488 KPL（紅色）染色的CD3標記細胞，使用BD FACS Calibur 在FL1通道上檢測。 | 圖3. 用fibronectin抗體標記人的心肌冰凍切片，并分別使用CF488A，Cy 2 和Alexa Fluor 488偶聯(lián)的羊抗兔IgG作為二抗染色。感謝來自德國Bad Nauheim (Hessen) Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut)的Sawa Kostin博士提供了上述圖片。 |
Table A. CF488A Dye-labeled Secondary Antibody Conjugates
| Cat# | Product Name | Unit Size | Unit Price | 品 牌 |
20289 | Bovine serum albumin, CF488A conjugate | 5x1mg | ￥1999 | 美國Biotium |
20293 | CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20293-1 | CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20225 | CF488A Chicken Anti-Goat IgG (H+L), 2mg/mL | 0.5 mL | ￥1764 | 美國Biotium |
20225-1 | CF488A Chicken Anti-Goat IgG (H+L), 2mg/mL | 50 uL | ￥764 | 美國Biotium |
20208 | CF488A Chicken Anti-Mouse IgG (H+L), 2mg/mL | 0.5 mL | ￥1764 | 美國Biotium |
20208-1 | CF488A Chicken Anti-Mouse IgG (H+L), 2mg/mL | 50 uL | ￥706 | 美國Biotium |
20209 | CF488A Chicken Anti-Rabbit IgG (H+L), 2mg/mL | 0.5 mL | ￥1764 | 美國Biotium |
20209-1 | CF488A Chicken Anti-Rabbit IgG (H+L), 2mg/mL | 50 uL | ￥706 | 美國Biotium |
20166 | CF488A Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20166-1 | CF488A Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL | 50 uL | ￥882 | 美國Biotium |
20016 | CF488A Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20016-1 | CF488A Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美國Biotium |
20169 | CF488A Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20169-1 | CF488A Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20074 | CF488A Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20074-1 | CF488A Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20014 | CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20014-1 | CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美國Biotium |
20015 | CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20015-1 | CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美國Biotium |
20027 | CF488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20027-1 | CF488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美國Biotium |
20024 | CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20024-1 | CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美國Biotium |
20020 | CF488A Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20020-1 | CF488A Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20017 | CF488A Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美國Biotium |
20017-1 | CF488A Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美國Biotium |
20022 | CF488A Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20022-1 | CF488A Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20010 | CF488A Goat anti-mouse IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美國Biotium |
20010-1 | CF488A Goat anti-mouse IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美國Biotium |
20011 | CF488A Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL | 0.25 mL | ￥1411 | 美國Biotium |
20302 | CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL | 0.5 mL | ￥2764 | 美國Biotium |
20302-1 | CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL | 50 uL | ￥1176 | 美國Biotium |
20018 | CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20018-1 | CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20246 | CF488A Goat anti-mouse IgG1, (?31), 2 mg/mL | 0.25mL | ￥2293 | 美國Biotium |
20256 | CF488A Goat anti-mouse IgG2a, (?32a), 2mg/mL | 0.25mL | ￥2293 | 美國Biotium |
20256-1 | CF488A Goat anti-mouse IgG2a, (?32a), 2mg/mL | 50 uL | ￥1000 | 美國Biotium |
20266 | CF488A Goat anti-mouse IgG2b, (?32b), 2mg/mL | 0.25mL | ￥2293 | 美國Biotium |
20012 | CF488A Goat Anti-Rabbit IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美國Biotium |
20012-1 | CF488A Goat Anti-Rabbit IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美國Biotium |
20013 | CF488A Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL | 0.25 mL | ￥1411 | 美國Biotium |
20019 | CF488A Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20019-1 | CF488A Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20023 | CF488A Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20023-1 | CF488A Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20028 | CF488A Goat Anti-Swine IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美國Biotium |
20028-1 | CF488A Goat Anti-Swine IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美國Biotium |
20228 | CF488A Monoclonal Mouse Anti-6X His IgG, 1mg/mL | 50 uL | ￥3352 | 美國Biotium |
20204 | CF488A Monoclonal Mouse Anti-Biotin, 2 mg/mL | 0.25 mL | ￥2117 | 美國Biotium |
20204-1 | CF488A Monoclonal Mouse Anti-Biotin, 2 mg/mL | 50 uL | ￥1000 | 美國Biotium |
20210 | CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL | 0.25 mL | ￥2293 | 美國Biotium |
20210-1 | CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL | 50 uL | ￥1117 | 美國Biotium |
20215 | CF488A monoclonal mouse anti-GFP IgG, 1 mg/mL | 0.1 mL | ￥2587 | 美國Biotium |
20079 | CF488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL | 0.5 mL | ￥1764 | 美國Biotium |
20079-1 | CF488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL | 50 uL | ￥706 | 美國Biotium |
20021 | CF488A Rabbit Anti-Goat IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美國Biotium |
20021-1 | CF488A Rabbit Anti-Goat IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美國Biotium |
20238 | CF488A Rabbit anti-HA (Hemagglutinin) IgG, 1 mg/mL | 50 uL | ￥3352 | 美國Biotium |
20071 | CF488A Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20071-1 | CF488A Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20026 | CF488A Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20026-1 | CF488A Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20025 | CF488A Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20025-1 | CF488A Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美國Biotium |
20172 | CF488A Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美國Biotium |
20172-1 | CF488A Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美國Biotium |
Table B. Other CF488A dye-labeled Products
| Cat# | Product Name | Unit Size | Unit Price | 品 牌 |
29005 | Annexin V, CF488A conjugate, 50 ug/mL | 0.5 mL | ￥2587 | 美國Biotium |
00005 | CF488A a-Bungarotoxin | 0.5 mg | ￥3528 | 美國Biotium |
29016 | CF488A Concanavalin A (Con A) | 5x1 mg | ￥1529 | 美國Biotium |
29034 | CF488A Streptavidin | 1 mg | ￥1882 | 美國Biotium |
00042 | Phalloidin, CF488A conjugate | 300 U | ￥3587 | 美國Biotium |
29022 | Wheat Germ Agglutinin, CF488A conjugate | 5x1mg | ￥2940 | 美國Biotium |
29022-1 | Wheat Germ Agglutinin, CF488A conjugate | 1mg | ￥706 | 美國Biotium |
Table C. Reactive CF488A Dyes and Labeling Kits
| Cat# | Product Name | Unit Size | Unit Price | 品 牌 |
92152 | CF488A hydrazide | 1 mg | ￥1764 | 美國Biotium |
92022 | CF488A maleimide | 1 umole | ￥2411 | 美國Biotium |
92213 | CF488A SE protein labeling kit | 3 labelings | ￥4292 | 美國Biotium |
92120 | CF488A succinimidyl ester | 1 umole | ￥2646 | 美國Biotium |
92051 | CF488A, aminooxy | 1 mg | ￥4586 | 美國Biotium |
92253 | Mix-n-Stain CF488A antibody labeling kit, 1x(20-50 ug) labeling | 1 labeling | ￥1235 | 美國Biotium |
92233 | Mix-n-Stain CF488A antibody labeling kit, 1x(50-100 ug) labeling | 1 labeling | ￥1352 | 美國Biotium |
92273 | Mix-n-Stain CF488A antibody labeling kit, 1X(5-20ug) labeling | 1 labeling | ￥1117 | 美國Biotium |
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