技術(shù)文章

基底硬度細(xì)胞牽張拉力影響，基底剛度細(xì)胞牽張拉伸刺激

閱讀：1098 發(fā)布時(shí)間：2019-12-26

基底硬度對(duì)細(xì)胞牽張拉力影響

CellSoft™ Soft Substrate References

Key References:

Buxboim et al., How deeply do cells feel:methods for thin gels. J Phys Condens matter. 22: 1-19, 2010.‍

Chodhury et al., Soft substrates promote homogeneous self-renewal of embryonic stem cells via down regulating cell-matrix tractions. PLOS 1 5: 1-10, 2010.‍

Engle et al., Matrix elasticity directs stem cell lineage specification. Cell 126: 677-689, 2006.

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Hirata and Yamaoka. Effect of stem cell niche elasticity/ECM protein on the self-beating cardiomyocyte differentiation of iPS cells at different stages. Acta Biomat. 65: 44-52, 2018

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Megone et al., Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterization of soft biomaterials. Sci Rep 8: 6780, 2018.

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Ou et al., Visualizing mechanical modulation of nanoscale organization of cell-matrix adhesions. Integr. Biol. 8: 795-804,2016.

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Palchesko et al., Development of polydimethylsiloxane substrates with tunable elastic modulus to study cell mechanobiology in muscle and nerve. PLOS 1 7: 1-13, 2012

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Smith et al., Mechanosensing of matrix by stem cells: from matrix heterogeneity, contractility and the nucleus in pore-migration to cardiogenesis and muscle stem cells in vivo. Sem Cell Devtbio. 71: 84-98, 2017

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Solon et al., Fibroblast adaptation andstiffness matching to soft elastic substrates. Biophys J. 93: 4453-4461, 2007.

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Thomas et al., Measuring the mechanical properties of living cells using atomic force microscopy. J Visual Exp. 76:1-8, 2013.

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Vertelov et al., Rigidity of silicone substrates controls cell spreading and stem cell differentiation. Sci Rep. 6:1-10, 2016.

flexcell FX-6000T基底硬度細(xì)胞應(yīng)力刺激加載系統(tǒng)

2D/3D細(xì)胞或組織靜態(tài)或動(dòng)態(tài)牽張等邊軸或單軸向牽張應(yīng)變加載和實(shí)時(shí)觀察
新增不用基底硬度牽張
FX-5000 Tension 系統(tǒng)組成部分:
• 一臺(tái)預(yù)裝FlexSoft®FX-5000細(xì)胞力學(xué)加載分析軟件的計(jì)算機(jī)
• 一臺(tái)FX5K™牽張拉伸應(yīng)力加載控制傳導(dǎo)儀
• 一個(gè)BioFlex真空腔應(yīng)力加載基板，四個(gè)密封墊片
• 一套BioFlex直徑為25mm的BioFlex應(yīng)力加載平臺(tái)
• 四塊BioFlex 膠原蛋白1型包被細(xì)胞培養(yǎng)板
• 一個(gè)干燥過濾器
• 一對(duì)濾水器
• 一卷25英尺藍(lán)色Flex In鏈接管(6.4毫米外徑)
• 一卷25英尺無色Flex Out鏈接管（9.5毫米外徑）
• 一卷25英尺牽張拉伸泵鏈接藍(lán)管(9.5毫米外徑）
• 一瓶硅膠潤(rùn)滑油
• 一臺(tái)儲(chǔ)壓穩(wěn)壓儀
• 一臺(tái)真空負(fù)壓牽張拉伸泵
牽張顯微附屬設(shè)備
倒置、正置即可
適用樣品：細(xì)胞或者組織（2D、3D）
軸向：二維等邊軸、二維單軸向、三維等邊軸、三維單軸向、三維梯形牽張
牽張模式：真空動(dòng)力抻拉培養(yǎng)板彈性柔性基底膜或3D水凝膠包埋的細(xì)胞
實(shí)時(shí)觀察：StageFlexer顯微附屬設(shè)備可在顯微鏡下觀察牽張作用下反應(yīng)
方便對(duì)照：可控制同一塊培養(yǎng)板部分孔細(xì)胞受力與否
多組牽張條件作用對(duì)照：可同時(shí)運(yùn)行多個(gè)不同壓力大小、不同頻率
不同加載周期程序，方便多組牽張條件對(duì)比；
軟件精準(zhǔn)調(diào)控：對(duì)牽張加載周期、張應(yīng)變大小、頻率、波形智能調(diào)控
牽張范圍：0 - 33%
牽張頻率：0.01- 5 Hz
細(xì)胞量大，便于后期分析：系統(tǒng)牽張傳導(dǎo)儀支持4塊6孔
（每孔1.2* 105個(gè)細(xì)胞）或24孔牽張板，可同時(shí)兼容4個(gè)獨(dú)立
的FlexLink牽張加載傳導(dǎo)儀，獨(dú)立操作四個(gè)不同的實(shí)驗(yàn)程序
支持任何波形種類：如更好地控制在超低或超高應(yīng)力下形：

●彈性模量范圍1-80kPa

●可選多孔板、60mm和100mm培養(yǎng)板

●BioFlex® CellSoft標(biāo)準(zhǔn)6孔板

●在柔性基底上牽拉細(xì)胞

●腔室載玻片CellSoft

●表面蛋白包被，無菌單獨(dú)包裝

CellSoft培養(yǎng)板有很多不同的種類，如不同的硬度，不同的孔板，用于顯微觀察的腔室載玻片（圓形多孔板），共價(jià)包被CollagenI或其他蛋白，可對(duì)細(xì)胞進(jìn)行靜態(tài)或動(dòng)態(tài)牽拉應(yīng)力刺激。更重要的一點(diǎn)，新型的CellSoft培養(yǎng)板可以反復(fù)胰酶消化和再接種細(xì)胞，蛋白包被的表面可以重復(fù)使用多達(dá)三次。