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技術(shù)文章

病毒核酸提取試劑盒

點擊次數(shù):2617 發(fā)布時間:2014-3-4

ABI公司病毒核酸提取試劑盒

MagMAX™-96 Viral RNA Isolation Kit

Description

Ambion's MagMAX™ Viral RNA isolation kits have been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. RNA isolation from cell-free samples presents some unique challenges compared to RNA isolation from tissues or cultured cells. These include low viral titers, large volumes to be processed, and sample complexity. Also, the procedure must be compatible with real-time qRT-PCR. The MagMAX™ Viral RNA isolation kits address all of these issues, without the need for organic solvents or RNA precipitation, eliminating the problems often encountered with filter-based RNA isolation methods such as clogging, large elution volumes, and inconsistent yields. 

• More effective recovery of viral RNA and DNA from diverse sample types 
• Improved qRT-PCR results 
• Ideal when working with low viral concentrations 
• Improved yields and reproducibility


MagMAX™-96 Viral RNA Isolation Kit
This high-throughput (96 well plate

Specifications

General Specifications

Sample Type (General):

Viral Samples, 

Liquid Samples (e.g. Serum) 

Sample Type (Specific):

Cell-Free Fluids 

Final Product:

Viral DNA⁄RNA 

Downstream Application:

Real-Time PCR, 

Northern Blotting, 

RT-PCR (Endpoint) 

Platform:

KingFisher™ Flex 

High Throughput Compatibility:

Manual Protocols, 

Automated Protocols, 

High Throughput-Compatible 

Isolation Technology:

Magnetic Bead 

Scale:

Midi 

Format:

96-well plate 

Product Size:

96 preps 

Yield (up to):

5 ng 

Purification Time:

45 min 

Number of Reactions:

96 Reactions 

Starting Material (Amount):

Up to 50µl of sample 

Regulatory Statement:

For Research Use Only. Not for use in diagnostic procedures. 

Contents and Storage

Carrier RNA is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, RNA Rebinding Concentrate, Bead ResuspensionSolution, Processing Plates and Lids, and PBS Buffer are stored at room temperature. Nuclease-free water may be stored at any temperature.

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