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Roche 11585614910DIG High Prime DNA Labeling and Detection Starter Kit II
【簡單介紹】
【詳細說明】
DIG High Prime DNA Labeling and Detection Starter Kit II
DIG High Prime DNA Labeling and Detection Starter Kit II|DNA高效地高辛標記及檢測試劑盒II|地高辛標記試劑盒|高效地高辛標記試劑盒|羅氏地高辛標記試劑盒
DNA高效地高辛標記及檢測試劑盒II (Roche 貨號: 11585614910)
DIG-High Prime DNA Labeling and Detection Starter Kit II
1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 μg per assay, blots of 100 cm2
Application
DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.
Product Description
Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
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電話:86-021-51875103
傳真:86-021-4006768800-5599
手機:15026509758
留言:發(fā)送留言
個性化:www.ybiotech.com.cn
手機站:m.ybiotech.com.cn
網(wǎng)址:www.yubiotech.com
商鋪:http://www.muquzhou.cn/st251076/