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BD 一抗 Ms F4/80 PE T45-2342 100ug 565410
貨號: 565410
品牌: BD Pharmingen
同種亞型: Rat WI, also known as Wistar (outbred) IgG2a, κ
反應(yīng)種屬: Mouse (QC Testing)
應(yīng)用: Flow cytometry (Routinely Tested)
BD 一抗 Ms F4/80 PE T45-2342 100ug 565410
Two-color flow cytometric analysis of F4/80 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with Alexa Fluor® 647 Rat Anti-Mouse CD11b (Cat. No. 557686) and either PE Rat IgG2a Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Mouse F4/80 antibody (Cat. No. 565410; Right Panel). The two-color flow cytometric contour plot showing the correlated expression of F4/80 (or Ig Isotype control staining) versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.