Recombinant Sarcoglycan Alpha (SGCa) 

肌糖α(SGCa)重組蛋白

[ PROPERTIES ]

Residues: Gln24~His387

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q16586

Host: E. coli

Subcellular Location: Cell Membrane; Single-passType I Membrane Protein. Cytoplasm.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 70.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH₂O..

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

QQTTLHP LVGRVFVHTL DHETFLSLPE HVAVPPAVHI TYHAHLQGHP DLPRWLRYTQRSPHHPGFLY GSATPEDRGL QVIEVTAYNR DSFDTTRQRL VLEIGDPEGP LLPYQAEFLVRSHDAEEVLP STPASRFLSA LGGLWEPGEL QLLNVTSALD RGGRVPLPIE GRKEGVYIKVGSASPFSTCL KMVASPDSHA RCAQGQPPLL SCYDTLAPHF RVDWCNVTLV DKSVPEPADEVPTPGDGILE HDPFFCPPTE APDRDFLVDA LVTLLVPLLV ALLLTLLLAY VMCCRREGRLKRDLATSDIQ MVHHCTIHGN TEELRQMAAS REVPRPLSTL PMFNVHTGER LPPRVDSAQVPLILDQH

[  IDENTIFICATION  ]

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