Recombinant Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1)

前膠原賴氨酸-1,2-酮戊二酸-5-雙加氧酶1(PLOD1)重組蛋白

[ PROPERTIES ]

Source:Prokaryotic expression

Host: E.coli

Residues:Gln636~Pro727

Tags:N-terminal His-Tag

Tissue Specificity: Liver, Kidney, Heart, Testis.

Subcellular Location: Rough endoplasmic reticulum membrane; Peripheralmembrane protein; Lumenal side.

Purity: > 95%

Traits:Freeze-dried powder

Buffer formulation: 100mM NaHCO₃, 500mM NaCl, pH8.3, containing1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration:200µg/mL

Applications:Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:9.1

Predicted Molecular Mass:14.2kDa

Accurate Molecular Mass:14kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentrationof0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

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