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上海橋星貿(mào)易有限公司
主營產(chǎn)品： 進(jìn)口透析袋,密理博ECL發(fā)光液,B27無血清培養(yǎng)基,Gibco膠原酶 |
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N2515 Recombinant RNasin Promega試劑?
原裝*，質(zhì)量保證，現(xiàn)貨供應(yīng)
規(guī)格：10000u
N2515 Recombinant RNasin Promega試劑? 產(chǎn)品描述：
其它組分：
Recombinant RNasin® Ribonuclease Inhibitor【子貨號(hào)：N251B，包裝：1 x 10,000u，，運(yùn)保溫度：–20°C】
描述：
說明 |
| 天然的和重組的RNasin® 核酸酶抑制劑具有廣譜的抑制RNase 活性的性質(zhì)，包括抑制中性型(neutral type) 真核RNase。該抑制劑的分子量為50kDa，按1:1 的比例與RNase 非共價(jià)結(jié)合發(fā)揮抑制作用。RNasin® Ribonuclease Inhibitor 與RNase( 如RNase A) 結(jié)合的Ki 值大約是10-14M。Promega 提供兩種不同的抑制劑：天然的RNasin® Ribonuclease Inhibitor 和重組的RNasin® Ribonuclease Inhibitor。兩種產(chǎn)品均通過離子交換和親和層析相結(jié)合的純化方法純化得到。
|
特點(diǎn) |
| • 抑制常見的真核RNase：RNasin® Ribonuclease Inhibitor 具有廣譜RNase 抑制特性，包括 RNase A、 RNase B、 RNase C 和人類胎盤RNase，不抑制RNase T1、 S1 核酸酶、曲霉(Aspergillus ) 中的 RNase 以及RNase H、RNase ONE® Ribonuclease。 |
原廠資料：
RNases are ubiquitous, cause RNA degradation and can compromise RNA integrity. Recombinant RNasin® Inhibitor is a 50kDa protein that inhibits RNase A family and human placental RNases by noncovalently binding to RNases in a 1:1 ratio. Recombinant RNasin® Inhibitor does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H, RNase ONE™ Ribonuclease and enzymes for downstream applications such as GoScript™ Reverse Transcriptase, AMV/M-MLV reverse transcriptases, SP6, T7/T3 RNA polymerase, and Taq DNA polymerases. Learn more about our custom options for this product at: www.promega。。ｃｏｍ/custom/
Features - Benefits
Broad-Spectrum RNase Inhibition: Inhibits common eukaryotic RNases.
Compatible: Does not inhibit SP6, T7 or T3 RNA Polymerase; GoScript™ Reverse Transcriptase, AMV or M-MLV Reverse Transcriptase; or Taq DNA polymerase.
Flexible: Functional across wide pH range (pH 5–8) enabling use in many downstream assays.
Recombinant: Made in bacteria, minimizing the chances of human nucleic acid contamination.
Applications
RT-PCR and RT-qPCR.
Useful in any applications where eukaryotic RNase contamination is a potential problem.
Protection of mRNA in cDNA synthesis reactions.
In vitro transcription/translation.
Increase yield and activity of polysomes.
Improvement of in vitro virus replication.
Improvement of RNA translation in homologous systems.
Performing RNase-free antibody staining.
Enhancement of RNA yields from Riboprobe® System RNA synthesis reactions.
References
Blackburn, P. and Moore, S. (1982) In: The Enzymes, Vol. XV, Part B.
Blackburn, P. et al. (1977) J. Biol. Chem. 252, 5904–10.
Lee, F.S. et al. (1989) Biochemistry 28, 219–24.
Lee, F.S. et al. (1989) Biochemistry 28, 225–30.
注意事項(xiàng)：
QC Tests
SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease.
Source
Recombinant strain of E. coli (Cat.# N2511, N2515).
Storage Buffer
20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT and 50% (v/v) glycerol.
Storage Conditions
Store at –20°C.
Unit Definition
One unit is defined as the amount of RNasin® Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.
For product intended use please see Patents & Disclaimers tab.
N2515 Recombinant RNasin Promega試劑? 相關(guān)產(chǎn)品列表：
| Promega 系列 | ||
| 序號(hào) | 貨號(hào) 名稱 | 規(guī)格 |
| 1 | M1801 T4連接酶 | 100U |
| 2 | M1804 T4連接酶 | 500U |
| 3 | M1701 M-MLV | 10000U |
| 4 | M1705 M-MLV | 50000U |
| 5 | M5101 AMV | 300U |
| 6 | M5108 AMV | 1000U |
| 7 | M7741 Pfu | 100U |
| 8 | M7745 Pfu | 500U |
| 9 | C1101 Olig(dt)15 Primer | 20ug |
| 10 | M3001 Go-Taq | 100U |
| 11 | M3005 Go-Taq | 500U |
| 12 | M3008 Go-Taq | 2500U |
| 13 | U1511 dNTP | 200μl |
| 14 | U1515 dNTP | 1000μl |
| 15 | A1360 pGEM-T easy | 1.2ug |
| 16 | A3600 pGEM-T | 1.2ug |
| 17 | C1181 Random Primers | 20μg |
| 18 | N2511 Recombinant RNasin | 2500U |
| 19 | N2515 Recombinant RNasin | 10000U |
| 20 | N2611 RNasin Plus | 2500U |
| 21 | N2615 RNasin Plus | 10000U |
| 22 | N2111 RNasin | 2500U |
| 23 | N2115 RNasin | 10000U |
| 24 | M1871 TdT | 300U |
| 25 | M1875 TdT | 1500U |
| 26 | M2825 CIAP | 1000U |
| 27 | M1821 CIAP | 1000U |
| 28 | M2201 Klenow I | 150U |
| 29 | M2206 Klenow I | 500U |
| 30 | M4211 T4 DNA Polymerase | 100U |
| 31 | M4215 T4 DNA Polymerase | 500U |
| 32 | M4101 T4 Polynucleotide Kinase | 100U |
| 33 | M4103 T4 Polynucleotide Kinase | 1000U |
