材料名稱

廠家

包裝規(guī)格/貨號(hào)

DMEM（干粉）

Invitrogen

1*10L

RPMI-1640 （干粉）

Invitrogen

1*10L

Opti-MEM I (液體)

Invitrogen

100mL

MEM （干粉）

Invitrogen

1*10L

MEM alpha （干粉）

Invitrogen

10*1L

Leibovitz’s L-15 (干粉)

Invitrogen

1*10L

DMEM/F12 (干粉)

Invitrogen

10*1L

EBM內(nèi)皮細(xì)胞培養(yǎng)基

Invitrogen

500mL

K-SFM培養(yǎng)基（液體）

Invitrogen

500mL

青霉素/鏈霉素

Invitrogen

100mL

Trypsin-EDTA

Invitrogen

100mL

Zeocin

Invitrogen

1G

Blasticidin

Invitrogen

10mg

L-Glutamine

Invitrogen

100mL

Insulin Transfrin-selenium

Invitrogen

10mL

REC. human EGF

Invitrogen

100ug

ESGRO (LIF)

Millipore

1mL

HEPES

Amresco

1KG

Leibovitz’sL-15 (液體，無(wú)酚紅)

Invitrogen

500mL

IMEM (液體，無(wú)酚紅)

Invitrogen

500mL

Sodium pyruvate

Hyclone

100mL

碳酸氫鈉

Sigma

500g

DMSO

MP

100mL

Charcoal Stripped FBS

HAKATA

50mL

Monoclonal anti-PSF

Sigma

200ul

Monoclonal anti-OCT4

Sigma

100ul

Anti-ATCB

Epitomics

100ul

Anti-FLAG

Sigma

1mg

Anti-CD34

Sigma

200ug

Anti-CCL18

Sigma

100ug

Anti-BRCA1

Sigma

100ug

Anti-BACH1

Sigma

100ul

Anti-N-Cadherin

Sigma

100ul

Anti-rabbit IgG, FITC

Sigma

200ug

Anti-mouse IgG, FITC

Sigma

200ug

Anti-goat IgG, FITC

Sigma

200ug

Endo-free plasmid mini kit

OMEGA

50T

Gel-extraction kit

OMEGA

50T

Plasmid miniprep

OMEGA

100T

RNA Probe purification Kit

OMEGA

5T

NEB迷你克隆套裝

NEB

1kit

ZERO BLUNT VECTOR

Invitrogen

40T

Cycle-Pure kit

OMEGA

100T

ECL試劑盒

Pierce

200mL

Dual Luciferase Reporter Assay system

Promega

1000T

動(dòng)物細(xì)胞/組織基因組DNA提取試劑盒

天根

50T

細(xì)胞凋亡檢測(cè)試劑盒

Promega

Kit

Protease inhibitor kit

Roche

Kit

病毒抽提試劑盒

QuickGene

Kit

蛋白抽提試劑盒

BestBio

Kit

LightShiftChemiluminescent RNA EMSA kit

Thermo

Kit

0.2mL超濾管

Millipore

個(gè)

Transwell板

Costar

24個(gè)/板

超低吸附培養(yǎng)板

Costar

包

FN（纖維粘連蛋白培養(yǎng)體系）

Costar

5mg

Faramount aqueous mounting medium

DAKO

15mL

DAPI

MP

10mg

Hochest

Sigma

100mg

Lipofectaine 2000 reagent

Invitrogen

1.5mL

Lipofectamin LTX

Invitrogen

1mL

TransIT-2020 transfection reagent

Mirusbio

1mL

96孔血清板

Costar

個(gè)

胰島素

Sigma

10mL

SsoFastevagreenSupermix

Biorad

5mL

Taq DNA polymerase

TaKaRa

250U

MLVReverse transcriptase

Invitrogen

20萬(wàn)U

PrimeSTAR HS DNA polymerase

TaKaRa

125U

RNase Inhibitor

Fermentas

2500U

RNase H

Fermentas

100U

Terminal transferase

NEB

500U

PolyU polymerase

NEB

60U

Protease

Sigma

50mL

Klenow Fragment

Fermentas

300U