詳細(xì)介紹
PRP-C18 瑞士 Hamilton 原裝PRP-C18 液相色譜柱
The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any phase or pH. The rigid stationary phase has excellent mechanical and thermal stability(> 100°C), does not shrink or swell, and is compley inert to most conditions commonly encountered in reversed-phase chromatography.
The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited for specialized applications in the clinical, pharmaceutical, environmental, food, forensics, and life sciences industries.
PRP-C18 瑞士 Hamilton 原裝PRP-C18 液相色譜柱
色譜柱參數(shù):
固定相 | PRP-C18 |
模式 | Reversed-Phase |
USP 代碼 | USP L21 |
填料類型 | PS-DVB functionalized with C18 |
填料粒度 | 5 μm |
孔徑 | 100 ? |
內(nèi)徑 | 4.6 mm |
柱管材質(zhì) | 不銹鋼 |
zui大耐壓 | 5,000 psi |
Phase Limits | pH 1-13. 0-100%水, 有機(jī)改性劑 |
緩沖液強(qiáng)度 | 0.0-0.5N |
溫度范圍 | 5-85°C |
柱子恢復(fù) | Run a gradient of 100% water to 100% acetonitrile. Repeat three times |
Compound Classification | Organics |
PRP-C18色譜柱-----在任意pH值均可實(shí)現(xiàn)高效分離
孔徑: 100A
材料:C18功能化的PS-DVB
在建立方法時(shí),改變流動(dòng)相的pH值是非常有效的,尤在分離一些中性胺類化合物或者在堿性條件下分離其他的有機(jī)堿類化合物。
盡管近年來有宣稱硅膠基C18色譜柱在堿性pH條件下能夠穩(wěn)定工作,但是所有的硅膠基C18色譜柱在pH>6時(shí),測(cè)量性能均有所下降,相比在理想條件下使用,色譜柱的使用壽命也顯著縮短。
另一方面,聚合物基質(zhì)色譜柱具有真正的化學(xué)穩(wěn)定性,固定相能夠長(zhǎng)期耐受濃度高達(dá)1M的NaOH和H2SO4 ,且測(cè)量性能不會(huì)下降。因?yàn)樵谌魏蝡H值下不會(huì)出現(xiàn)填料脫落,滲出或溶解現(xiàn)象,所以色譜柱無需考慮流動(dòng)相狀況在整個(gè)使用壽命內(nèi)均可可靠地重復(fù)檢測(cè)。
高pH值的應(yīng)用
目前超過70%的藥物成分為陽離子溶質(zhì),在pH<7時(shí),攜帶正電荷,過去一直很難將其與其他有機(jī)堿分離。主要是因?yàn)殡x子化在反相色譜法中起決定性作用,電離胺樣品的洗脫窗口十分狹窄;其次帶正電荷的溶質(zhì)和色譜柱固定相上殘余的硅醇基發(fā)生第二次相互作用,常常導(dǎo)致出現(xiàn)峰值形狀不佳,保留時(shí)間出現(xiàn)漂移和柱效降低等異常現(xiàn)象,更進(jìn)一步縮短色譜柱使用壽命。而PRP-C18可以解決這些問題。
快速洗脫
在現(xiàn)代藥物研發(fā)科學(xué)中,常規(guī)分析色譜不應(yīng)該成為瓶頸。趨勢(shì)是使用包含較小顆粒的較短色譜柱并在較高流速下使用,從而提高生產(chǎn)率。PRP-C18目前*要求 。
訂貨信息:
訂貨號(hào) | 產(chǎn)品描述 |
79477 | Analytical Guard Cartridge Holder, PEEK |
32908 | Analytical Guard Cartridge Holder, Stainless Steel |
79291 | Analytical Guard Column, PRP-C18 |
79641 | Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID) |
79643 | Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID) |
79644 | Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID) |
79645 | Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID) |
79675 | PRP-C18 5 μm 4.6 x 50 mm |
79678 | PRP-C18 12-20 μm 21.2 x 250 mm |
79673 | PRP-C18 5 μm 2.1 x 150 mm |
79680 | PRP-C18 5 μm 2.1 x 150 mm PEEK |
79674 | PRP-C18 5 μm 2.1 x 250 mm |
79681 | PRP-C18 5 μm 2.1 x 250 mm PEEK |
79672 | PRP-C18 5 μm 2.1 x 50 mm |
79679 | PRP-C18 5 μm 2.1 x 50 mm PEEK |
79676 | PRP-C18 5 μm 4.6 x 150 mm |
79683 | PRP-C18 5 μm 4.6 x 150 mm PEEK |
79677 | PRP-C18 5 μm 4.6 x 250 mm |
79684 | PRP-C18 5 μm 4.6 x 250 mm PEEK |
79682 | PRP-C18 5 μm 4.6 x 50 mm PEEK |
79688 | PRP-C18 Analytical Guard Column Replacement Cartridges (5/pk), PEEK |
79686 | PRP-C18 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel |
79687 | PRP-C18 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK |
79685 | PRP-C18 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel |
79690 | PRP-C18 Semiprep/Preparative Guard Column Replacement Cartridges (2/pk), Stainless Steel |
79689 | PRP-C18 Semiprep/Preparative Guard Column Starter Kit (1 holder, 1 cartridge), Stainless Steel |
79792 | PRP-C18, 10 μm Bulk Resin |
79793 | PRP-C18, 12-20 μm Bulk Resin |
79791 | PRP-C18, 5 μm Bulk Resin |
5095-01 | Semiprep/Preparative Guard Cartridge Holder, Stainless Steel |
79918 | Semiprep/Preparative Guard Column, PRP-C18 |
79642 | Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID) |