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詳細(xì)介紹

概述
描述	Recombinant Human C-X3-C Motif Chemokine 1 is produced by our Mammalian expression system and the target gene encoding Gln25-Arg339 is expressed with a 6His tag at the C-terminus.
別名	Fractalkine, C-X3-C Motif Chemokine 1, CX3C Membrane-Anchored Chemokine, Neurotactin, Small-Inducible Cytokine D1, CX3CL1, FKN, NTT, SCYD1
形態(tài)	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
來(lái)源	Human Cells
氨基酸序列	QHHGVTKCNITCSKMTSKIPVALLIHYNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHL DRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTS PELPTGVTGSSGTRLPPTPKAQDGGPVGFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAP STQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMA HVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATRVDHHHHHH
內(nèi)毒素水平	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Accession #	P78423

性能
背景	Human Fractalkine (CX3CL1) is a member of the CX3C family of chemokines. Human Fractalkine contains both chemokine and mucin domain. The soluble form of Fractalkine is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane bound form of Fractalkine promotes leukocytes adhesion to endothelial cells. Fractalkine regulates leukocyte adhesion and migration processes at the endothelium and binds to CX3CR1. Natural Human Fractalkine is produced as a long protein (373-amino acid). The mucin-like stalk permits it to bind to the cell surface.
溶解方法	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存方法	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
純度	Greater than 95% as determined by reducing SDS-PAGE.