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詳細(xì)介紹

概述
描述	Recombinant Human Interleukin-1 beta is produced by our E.coli expression system and the target gene encoding Ala117-Ser269 is expressed.
別名	Interleukin-1 beta, Catabolin, IL1F2, IL1B.
形態(tài)	Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
來(lái)源	E. coli
氨基酸序列	MAPVRSLNCTLRDSKSLVMSGPYELKALHLQGQDMEVVFSMSFVQGEESNDKIPVALGLKE KNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAEN MPVFLGGTKGGQDITDFTMQFVSS
內(nèi)毒素水平	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Accession #	P01584

性能
背景	IL1B belongs to the IL-1 family. Interleukin 1 (IL-1) is a family of polypeptide cytokines consisting of two agonists, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2) encoded by two distinct genes and perform identical biological functions. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response. It is identified as endogenous pyrogens, and is reported to stimulate the release of prostaglandin and collagenase from synovial cells.
溶解方法	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存方法	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
純度	Greater than 95% as determined by reducing SDS-PAGE.