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Contents and Storage

• 3-7 x 106A549-ASCoV2-NLRP1 cells in a cryovial or shipping flask.

IMPORTANT: If cells provided in a cryovial are not frozen upon arrival, contact InvivoGen immediately.

• 1 ml of Blasticidin(10 mg/ml). Store at 4°C or at -20°C.*

• 1 ml of Normocin™ (50 mg/ml), a formulation of three antibiotics active against mycoplasmas, bacteria and fungi. Store at -20°C.*

*The expiry date is specified on the product label. Handling Frozen Cells Upon Arrival Cells must be thawed immediately upon receipt and grown according to handling procedures (as described on the next page) to ensure the best cell viability and proper assay performance. Note: Avoid freezing cells upon receipt as it may result in irreversible damage to the cell line.

Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedures.

IMPORTANT: For cells that arrive in a shipping flask please refer to the enclosed ‘cell recovery procedure’.

Cell Line Stability

Cells will undergo genotypic changes resulting in reduced responsiveness over time in normal cell culture conditions. Genetic instability is a biological phenomenon that occurs in all stably genetically engineered cells. Therefore, it is critical to prepare an adequate number of frozen stocks at early passages. To ensure maximum efficiency, do not passage A549-ASCoV2-NLRP1 cells more than 20 times.

Quality Control

• The induction of ASC::GFP expression has been verified by western blot and fluorescence microscopy.

• The overexpression of the human ACE2 (hACE2) gene has been verified by flow cytometry and functional assays.

• The overexpression of the human TMPRSS2 (hTMPRSS2) gene has been verified by RT-qPCR and functional assays.

• The overexpression of the human NLRP1 (hNLRP1) gene has been verified by western blot and functional assays.

• The stability of this cell line for 20 passages following thawing has been verified.

• A549-ASCoV2-NLRP1 cells are guaranteed mycoplasma-free.

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北京百奧創(chuàng)新科技有限公司

北京百奧創(chuàng)新科技有限公司

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