精品国产亚洲国产亚洲,久热中文在线观看精品视频,成人三级av黄色按摩,亚洲AV无码乱码国产麻豆

武漢原生原代生物醫(yī)藥科技有限公司

PriCells: Primary dermal fibroblasts isolated from human

時間:2021-10-29 閱讀:209
分享:

PriCells: Primary dermal fibroblasts isolated from human                  


Fibroblasts were established from donors of human normal skin, scar, and keloid using the explant method.

The specimens were washed with sterile phosphate-buffered saline (PBS), and the subcutaneous tissues were removed carefully.

The skins were then cut into small pieces (1-2 mm3).

After being digested with PriCells Isolation Kit III (or using 0.25% trypsin containing 0.05% ethylenediaminetetraacetic acid) at 4°C overnight, the epidermal layers were removed, and the remaining dermal parts were further digested with PriCells Isolation Kit III (or using 0.25% trypsin containing 0.05% ethylenediaminetetraacetic acid) at 37°C for another 4 hours.

The digested cells were then passed through a 70-µm cell strainer (BD Biosciences), centrifuged, and resuspended in PriCells primary cell culture system.

Cells were seeded on tissue culture plates at 1x103 cells/cm2, and maintained at 37°C with 5% CO2.

After 24 hours, the plates were washed with PBS to remove residual non-adherent cells.

The resulting adherent cells were grown to confluency within 7 days.

Cells were subcultured every 4 days using 0.25% trypsin/EDTA.


會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~
撥打電話 產(chǎn)品分類
在線留言
欧美亚洲另类天天综合网| 视频一区二区三区日韩视频| 在线免费观看一区二区三区| 欧美人人做人人爽人人喊| 日本高清不卡一区二区三区| 手机成人三级a在线观看| 国产乱精品一区二区三区视频了| 久久国产老熟女老女人| 久久久精品亚洲Av| 奇米一区二区三区视频在线观看| 操逼动漫首页登录| 涩涩屋操美女视频| 加勒比五月综合久久伊人| 人妻人久久精品中文字幕| 老熟妇高潮一区二区高清视频| 亚洲一区亚洲二区在线观看| 被大鸡巴操淫液视频| 大鸡巴抽插小骚逼视频免费| 小穴抽插流水视频| 亚洲一区亚洲二区在线观看| 日韩在线视频不卡一区二区三区| 女人被男人操到高潮视频| 加勒比在线不卡一区二区观看| 国产品无码一区二区三区在线| 伊人久久亚洲婷婷综合久久| 男女边吃奶边做边爱视频| 美女主播被操流水| 成人av大全免费一区二区三区| 黄色视频力肏女人| 把美女日到高潮喷水视频| 久久久三级黄片免费视频| 欧美区 日韩区 亚洲区| 欧美精品一区二区三区四区五区| 日韩人妻无码中字一区二区| 欧美日本欧美日本区一区二| 少妇毛片一区二区三区免费视频| 日本免费精品一区二区三区四区| 被下药强奷到舒服的视频| 波多野结衣浴尿解禁在线| 色噜噜噜噜一区二区三区| 我要操日本女人的逼|