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Axion CytoSMART Exact細(xì)胞計(jì)數(shù)儀，是一款荷蘭進(jìn)口全自動(dòng)細(xì)胞計(jì)數(shù)儀器，無論是在明場(chǎng)還是熒光通道下，都可以高效、準(zhǔn)確地評(píng)估2D/3D細(xì)胞濃度和活力，獲得精準(zhǔn)可重復(fù)的實(shí)驗(yàn)結(jié)果。由于使用了新型的光學(xué)和圖像分析軟件技術(shù)，可實(shí)現(xiàn)自動(dòng)對(duì)焦，3s完成細(xì)胞計(jì)數(shù)，可以區(qū)分細(xì)胞及其碎片、識(shí)別出細(xì)胞團(tuán)中的個(gè)體、計(jì)算類器官的大小和數(shù)量等。



 
CytoSMART Exact細(xì)胞計(jì)數(shù)儀應(yīng)用

1、各類細(xì)胞的全自動(dòng)細(xì)胞計(jì)數(shù)、活率分析、細(xì)胞大小分析：

明場(chǎng)利用臺(tái)盼藍(lán)染色劑、或雙熒光通道使用綠色/紅色熒光染色劑（AO/PI雙染），可以計(jì)算細(xì)胞總數(shù)（細(xì)胞數(shù)/mL）、活細(xì)胞及死細(xì)胞數(shù)量（細(xì)胞數(shù)/mL）、細(xì)胞活力(%)、活細(xì)胞及死細(xì)胞的大?。?mu;m）?？蓪?shí)現(xiàn)從細(xì)胞團(tuán)中分辨單個(gè)細(xì)胞，通過細(xì)胞大小門控設(shè)定來選擇細(xì)胞亞群，了解樣本內(nèi)細(xì)胞大小分布情況。
 

 

2、球形和不規(guī)則形狀類器官的計(jì)數(shù)、大小、群體分布分析：

類器官計(jì)數(shù)模塊采用了新型AI分析算法，能夠快速精準(zhǔn)地識(shí)別不同形狀的類器官，在計(jì)算樣本數(shù)量的同時(shí)，給出大小分布。軟件通過“總覽”、“類器官濃度”、“類器官面積”，從不同角度和時(shí)間維度來展示受測(cè)樣本的特征?？梢赃x擇手動(dòng)或自動(dòng)設(shè)定細(xì)胞大小門控，了解樣本中類器官在不同時(shí)間點(diǎn)的濃度及平均大小，或是不同尺寸類器官在數(shù)量上的分布規(guī)律，將多個(gè)時(shí)間點(diǎn)的分析數(shù)據(jù)歸納到一起比較，還可以掌握類器官數(shù)量變化對(duì)于樣本總體細(xì)胞濃度的影響。




 

CytoSMART Exact細(xì)胞計(jì)數(shù)儀優(yōu)勢(shì)

1、快速計(jì)數(shù)，高準(zhǔn)確率

全自動(dòng)對(duì)焦，3s完成細(xì)胞計(jì)數(shù)；采用高倍率、高對(duì)比度光學(xué)組件，640萬CMOS成像，高分辨率有效區(qū)分細(xì)胞和碎片；更大視野，可實(shí)現(xiàn)同一樣本8個(gè)區(qū)域拍照取均值；使用電腦鏈接國(guó)內(nèi)云端服務(wù)器，進(jìn)行圖像分析和儲(chǔ)存，通過創(chuàng)新的機(jī)器學(xué)習(xí)軟件，大幅提高了實(shí)驗(yàn)的準(zhǔn)確性和可重復(fù)性，并有效降低了人工計(jì)數(shù)時(shí)不同人員操作差異對(duì)結(jié)果的影響。

2、熒光計(jì)數(shù)，細(xì)胞亞群分析

除通過明場(chǎng)獲得細(xì)胞總數(shù)量、活力外，還可通過紅色熒光、綠色熒光通道計(jì)算樣本中特定類群細(xì)胞的數(shù)量。

3、使用方便

操作簡(jiǎn)單、無需安裝、無需校準(zhǔn)、無需維護(hù)，任何實(shí)驗(yàn)室都能快速上手開始實(shí)驗(yàn)。

4、兼容各品牌細(xì)胞計(jì)數(shù)板

兼容市面上各類透明、深度為0.1mm計(jì)數(shù)板，不論塑料、還是玻璃材質(zhì)，不論有無記數(shù)格，不論有幾個(gè)計(jì)數(shù)腔室，不論是重復(fù)使用還是一次性計(jì)數(shù)板，均可用于CytoSMART Exact。

 

參考文獻(xiàn)

1.Migration of Myogenic Cells Is Highly Influenced by Cytoskeletal Septin7. Zsolt Ráduly, László Szabó, Beatrix Dienes, et al. Cells, 2023, 12(14), 1825, https://doi.org/10.3390/cells12141825
2. MicroRNA-375 repression of Kruppel-like factor 5 improves angiogenesis in diabetic critical limb ischemia. Michael G. McCoy, Anurag Jamaiyar, Grasiele Sausen, et al. Angiogenesis, Volume 26, Pages 107–127 (2023)
3. Deacetyl epoxyazadiradione ameliorates BPA-induced neurotoxicity by mitigating ROS and inflammatory markers in N9 cells and zebrafish larvae. Raghul Murugan, B. Haridevamuthu, Rajendran Saravana Kumar, et al. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, Volume 271, September 2023, 109692
4. Cell shape characteristics of human skeletal muscle cells as a predictor of myogenic competency: A new paradigm towards precision cell therapy. Charlotte Desprez, Davide Danovi, Richard M Day, et al. Journal of Tissue Engineering, March 16, 2023, https://doi.org/10.1177/2041731422113979
5. An In Vitro Study of the Healing Potential of Black Mulberry (Morus nigra L.) Extract in a Liposomal Formulation. Adriana Ramona Memete, Florina Miere (Groza) 2, Vasile Laslo, et al. Applied Sciences, 2023, 13(2), 1041, https://doi.org/10.3390/app13021041
6. Osteogenesis and osteoclastogenesis on a chip: Engineering a self-assembling 3D coculture. M.A.M. Vis, F. Zhao, E.S.R. Bodelier, et al. Bone, Volume 173, August 2023, 116812
7. Printing channels with millimeter-scale curvature and deciphering their effect on the proliferation, morphology, orientation, and migration of M-22 cells. Huinan Lai, Yuye Huang, Jun Yin, Jin Qian. Int J Bioprint. 2023; 9(3): 681
8. Highly Pluripotent Adipose-Derived Stem Cell–Enriched Nanofat: A Novel Translational System in Stem Cell Therapy. Lindsey Alejandra Quintero Sierra, Reetuparna Biswas, Francesco De Francesco, et al. Cell Transplantation, May 27, 2023, https://doi.org/10.1177/09636897231175968
9. Toxicity evaluation of Pinus radiata D.Don bark wax for potential cosmetic application. Daniel Sandoval-Rivas, Daniela V. Morales, Matías I. Hepp. Food and Chemical Toxicology, Volume 178, August 2023, 113896
10. Chitin-Glucan Complex Hydrogels: Physical-Chemical Characterization, Stability, In Vitro Drug Permeation, and Biological Assessment in Primary Cells. Diana Araújo, Thomas Rodrigues, Catarina Roma-Rodrigues, et al. Polymers, 2023, 15(4), 791, https://doi.org/10.3390/polym15040791

 


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