Cryopreserved vial (10 x 10^6 cells) of CD4⁺ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD8⁺ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD4⁺ T cells via immunomagnetic separation. Before and after CD4⁺ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell #07930) at a controlled rate.

Source
Normal human PBMC from Leukapheresis Sample