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當(dāng)前位置:BPS Bioscience Inc.>>Cell Signaling Pathway Cell Lines>> 78581RFP/GFP Safe-Harbor HEK293 Cell Line
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產(chǎn)品型號(hào)78581
品 牌BPS Bioscience
廠商性質(zhì)生產(chǎn)商
所 在 地
更新時(shí)間:2023-11-11 17:48:39瀏覽次數(shù):174次
聯(lián)系我時(shí),請(qǐng)告知來自 化工儀器網(wǎng)AP-1 Reporter – HEK293 Recombinant
CEACAM5 CHO Cell Line (High, Medium
ARE Luciferase Reporter HepG2 Cell
Claudin-6 CHO Cell Line (High, Medi
Recombinant stable HEK293 cell line constitutively expressing RFP (Red Fluorescent Protein) and GFP (Green Fluorescent Protein), which have been stably integrated into the AAVS1 safe harbor locus on chromosome 19.
Expression of RFP is driven by a CMV promoter, whereas GFP is driven by an EF1A promoter. The combined DNA fragment (CMV-RFP-bGH Poly A-EF1A-GFP-T2A-Puro-SV40 Poly A) (Figure 1) was integrated at the AAVS1 safe harbor locus using CRISPR/Cas9 technology. Cells were cloned by limiting dilution to obtain a monoclonal population. Without the adverse effects resulting from random integrations of RFP-GFP into the HEK293 genome, this cell line behaves like parental HEK293 cells.
Figure 1: Transgene integration at the AAVS1 locus.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
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