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1,10-Phenanthroline, Monohydrate
A20, His tag, FLAG-tag (Sf9-derived
The C-CBL-driven AXL Ubiquitination Intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin Donor and a Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this assay does not detect mono-ubiquitination. The FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses of polyubiquitination.
Figure 1: C-CBL-driven AXL ubiquitination intrachain TR-FRET Assay Kit schematic.
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