在2017年12月27日，由Jing Xu，F(xiàn)uhua Xu，Maralee S Lawson，Olena Y Tkachenko等作者在Biology of Reproduction中發(fā)表一篇了《Anti-Mullerian hormone is a survival factor and promotes the growth of rhesusmacaque preantral follicles during matrix-free culture》的文章。

文章使用Revolve正倒置一體顯微鏡獲得了如下結(jié)果:

該圖中B-E是通過Revolve正倒置一體顯微鏡成像得到的結(jié)果 ↑

該圖中A-B、C-F是通過Revolve正倒置一體顯微鏡成像得到的結(jié)果 ↑

中文摘要：

抗穆勒氏管激素（AMH）在卵泡發(fā)育過程起著至關重要的作用，其局部作用于卵泡生長過程中的動態(tài)分泌有關。目前不同物種的AMH在腔內(nèi)卵泡生長和功能的影響已有所驗證，但對腔前卵泡發(fā)育的作用仍待進一步驗證。因此，我們通過實驗研究AMH在腔前卵泡發(fā)育過程中所起到的作用。利用AMH-shRNA重組腺病毒載體降低獼猴卵泡中AMH基因的表達。將次生卵泡從成年獼猴卵巢中分離出來后用超低附著培養(yǎng)皿單獨培養(yǎng)，且培養(yǎng)基中加入促卵泡激素和胰島素，培養(yǎng)5周。將卵泡隨機分配給治療組，分別為a.對照組；b.非靶向?qū)φ战MshRNA-vector；c.AMH shRNA-vector；d.AMH shRNA-vector + 重組人源AMH；e.重組人源AMH。評估卵泡的存活和生長情況，并分析培養(yǎng)基中的類固醇激素和旁分泌因子的濃度。在體內(nèi)研究中，將非靶向?qū)φ誷hRNA-vecotor和AMH shRNA-vector分別注入獼猴的卵巢，術后9天對卵巢進行形態(tài)學及免疫組化檢測。結(jié)果發(fā)現(xiàn)，在非人靈長類動物中，AMH表達下調(diào)抑制腔前卵泡的存活和生長。體外實驗發(fā)現(xiàn)，在培養(yǎng)基中添加AMH則可促進腔前卵泡生長發(fā)育至小腔竇期，類固醇激素和旁分泌因子表達增加，并促進卵母細胞成熟。這些數(shù)據(jù)表明，AMH是一個關鍵的卵泡旁分泌/自分泌因子，促進靈長類的腔前卵泡存活和生長。

英文摘要：

Anti-Mullerian hormone (AMH) plays a key role during ovarian follicular development, with local actions associated with a dynamic secretion profile by growing follicles. While results for AMH effects on antral follicle growth and function are consistent among studies in various species, any effects on preantral follicle development remain controversial. Therefore, experiments were conducted to investigate the direct actions and role of AMH during follicle development at the preantral stage. Macaque-specific short-hairpin RNAs (shRNAs) targeting AMH mRNA were incor- porated into adenoviral vectors to decrease AMH gene expression in rhesus macaque follicles. Secondary follicles were isolated from adult macaque ovaries and cultured individually in the ultra-low-attachment dish containing defined medium supplemented with follicle-stimulating hor- mone and insulin for 5 weeks. Follicles were randomly assigned to treatment groups: (a) control,(b) nontargeting control shRNA-vector, (c) AMH shRNA-vector, (d) AMH shRNA-vector + recombi-nant human AMH, and (e) recombinant human AMH. Follicle survival and growth were assessed. Culture media were analyzed for steroid hormone and paracrine factor concentrations. For in vivo study, the nontargeting control shRNA-vector and AMH shRNA-vector were injected into macaque ovaries. Ovaries were collected 9 days postinjection for morphology and immunohistochemistry assessment. Decreased AMH expression reduced preantral follicle survival and growth in non- human primates. Supplemental AMH treatment in the culture media promoted preantral follicle growth to the small antral stage in vitro with increased steroid hormone and paracrine factor production, as well as oocyte maturation. These data demonstrate that AMH is a critical follicular paracrine/autocrine factor positively impacting preantral follicle survival and growth in primates.

ECHO Revolve正倒置一體顯微鏡展現(xiàn)了其非凡的靈活性，可以輕松地實現(xiàn)正置和倒置顯微鏡轉(zhuǎn)換，創(chuàng)新性地把正倒置顯微鏡合二為一，開啟了顯微鏡Hybrid時代。

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ECHO Revolve正倒置一體顯微鏡兼具正置和倒置顯微鏡的優(yōu)勢，方便小巧，一機多能，可以非常便利地通過旋轉(zhuǎn)實現(xiàn)正倒置配置切換；以iPad桌面替代目鏡設計, 獲得的視覺體驗；同時基于iOS的Echo app，使軟件操作更人性化。