訂貨：，，

1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCl  （EDC）介紹

Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCl  （EDC）現(xiàn)貨供應(yīng)

1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCl  （EDC）品牌介紹

美國Pierce公司（www.pierce.com）是蛋白質(zhì)化學、免疫學和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要產(chǎn)品包括：

1）化學發(fā)光體和比色底物

2）分析試劑

3）熒光標記探針

4）酶聯(lián)免疫分析試劑盒等

1-ETHYL-3-（3-DIMETHYLAMINOPROPYL）CARBODIIMIDE.HCl  （EDC）僅用于科研

Thermo Scientific Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.

1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride （EDC or EDAC） is a zero-length crosslinking agent used to couple carboxyl groups to primary amines. This crosslinker has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5’ phosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide （Sulfo-NHS）, EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding Sulfo-NHS.

Highlights:

• Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
• By combining EDC and Sulfo-NHS, amine reactive Sulfo-NHS esters can be created on any carboxyl-containing molecule
• Amide bonds formed with this crosslinking reaction provide a neutral linkage
• Excess reagent and crosslinking byproducts are easily removed by washing with water or dilute acid
• EDC is water soluble, so crosslinking can be done in physiologic solutions without adding organic solvent
• High purity, crystalline EDC can be used to create high-purity activated derivatives

• 佰易聚生物商城提供優(yōu)質(zhì)的分子生物學試劑和試劑盒、美國聯(lián)合碳化和美國光譜醫(yī)學透析袋、培養(yǎng)基和培養(yǎng)耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄www.ebioeasy.com或者,，。  洽談選購！