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CYT-448

重組人Myostatin Propeptide（rHuGDF-8）

5ug/25ug

Prospec

Introduction:
	Myostatin （GDF-8）, a member of the TGFbeta superfamily, is a potent and specific negative regulator of skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority （>70%） of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo.
	Description:
	28 kDa protein containing 243 amino acid residues of the human Myostatin Propeptide and 5 additional amino acid residues. MG NENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRRKLN
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Specificity:
	The amino acid sequence of the recombinant human Myostatin Propeptide is 100% homologous to the amino acid sequence of the human Myostatin Propeptide without signal sequence.
	Purification Method:
	Two-step procedure using size exclusion chromatography before and after refolding.
	Purity:
	>95% （SDS-PAGE analyzed）.
	Formulation:
	Lyophilized from a concentrated （1mg/ml） solution containing 0.5 mg/ml in 20mM TRIS, 20mM NaCl, pH 8.0.
	Solubility:
	Add 0.2 ml of d H₂O and let the lyophilized pellet dissolve compley.
	Stability:
	Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time. The lyophilized protein remains stable until the expiry date when stored at -20°C.
	Applications:
	Western blotting, ELISA
	References:
	• Jiang MS, Liang LF, Wang S, Ratovitski T, Holmstrom J, Barker C, Stotish R. Characterization and identification of the inhibitory domain of GDF-8 propeptide. Biochem Biophys Res Commun. 2004 Mar 12;315（3）:525-31. • Hill JJ, Davies MV, Pearson AA, Wang JH, Hewick RM, Wolfman NM, Qiu Y. The myostatin propeptide and the follistatin-related gene are inhibitory binding proteins of myostatin in normal serum. J Biol Chem. 2002 Oct 25;277（43）:40735-41. Epub 2002 Aug 22. • Thies RS, Chen T, Davies MV, Tomkinson KN, Pearson AA, Shakey QA, Wolfman NM. GDF-8 propeptide binds to GDF-8 and antagonizes biological activity by inhibiting GDF-8 receptor binding. Growth Factors. 2001;18（4）:251-9. • Lee SJ, McPherron AC. Regulation of myostatin activity and muscle growth. Proc Natl Acad Sci USA. 2001 Jul 31;98（16）:9306-11. Epub 2001 Jul 17.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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