美國NOVABIOS乙型腦炎病毒檢測試劑盒用于暴露于日本腦炎病毒（JEV）是用于檢測人血清中的IgE抗體對JEV衍生的重組抗原（JERA）（1-4）的ELISA測定系統(tǒng)。這個測試是為了幫助診斷人類暴露于日本腦炎病毒（JEV）。它不是為了篩選血液或血液成分，僅用于專業(yè)的體外診斷用途。該試劑盒尚未針對疫苗誘導(dǎo)的血清學(xué)轉(zhuǎn)換研究進行優(yōu)化。

美國NOVABIOS乙型腦炎病毒檢測試劑盒

 

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JE Detect^TM IgM ANTIBODY

CAPTURE ELISA (MAC-ELISA)

JE Detect MAC-ELISA-specific materials:

 

• Anti-Human IgM Coated Microtiter Strips: Strip holder with cover, containing 96 polystyrene

美國NOVABIOS乙型腦炎病毒檢測試劑盒

microtiter wells coated with antibody to human IgM in each well. Store at 2-8ºC until ready to use. The anti-human IgM coated wells are used to capture IgM antibodies from human samples.

• Sample Dilution Buffer for JE DetectIgM: One bottle, 25 mL, for ELISA plate wells in IgM assay. Store at –2-8º C until ready to use.

 

Note: If any precipitate is seen, vortex the tube very well to obtain a homogeneous solution and then use.

 

• Ready-to-use JE Antigen (JERA) for IgM: One tube (3 ml) of Ready-to-use JERA solution. Store at

–2-8º C until ready to use.

• Ready-to-use normal cell antigen (NCA) for IgM: One tube (3 ml) of Ready-to-use NCA solution. Store at –2-8º C until ready to use.
• JE Detect Negative Control: One vial, 30 µL of heat–inactivated serum. The JE Detect Negative Control will aid in monitoring the integrity of the kit as well. Store at 2-8°C until ready to use for up to 7 days. Quick spin the vial briefly before use to collect the content at the bottom.

Note: For long-term storage, serum can be further aliquoted in a smaller volume and stored at –70°C.

• JE Detect IgM Positive Control: One vial, 30 µL of heat–inactivated serum. The JE Detect IgM Positive Control will aid in monitoring the integrity of the kit as well. Store at 2-8°C until ready to use for up to 7 days. Quick spin the vial briefly before use to collect the content at the bottom.

Note: For long-term storage, serum can be further aliquoted in a smaller volume and stored at –70°C.

• 10X Wash Buffer: One bottle, 120 mL of Wash Buffer to be used in all the washing steps of this procedure. Store at 2-8°C until ready to use.
• Ready to Use Enzyme Conjugate-HRP for IgM: One bottle, 6 mL of a pre-diluted HRP conjugated flavivirus reactive monoclonal antibody (mAb) to be used as is in the procedure below. Store at 2-8°C until ready to use.
• EnWash: One bottle, 20 mL of EnWash to be used in between the washing steps after the addition of enzyme conjugate-HRP of this procedure. Store at 2-8º C until ready to use.

 

• Liquid TMB Substrate: One bottle, 9 mL of liquid substrate to be used in this procedure. Store at 2-8ºC until ready to use.

 

Note: The substrate should be kept in a light -protected bottle at all times as provided.

11. Stop Solution: One bottle, 6 mL to be used to stop the reaction. Store at 2-8°C until ready to use.

 

Caution: strong acid, wear protective gloves, mask and safety glasses. Dispose of all materials according to safety rules and regulations.

 

更具體的說明請根據(jù)以下信息

 

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【公司名稱】 廣州健侖生物科技有限公司
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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室