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用于使用豬瘟E2亞單位疫苗的豬場(chǎng)凈化豬瘟，鑒別野毒感染抗體和疫苗抗體使用。

For detection of antibodies to the Erns protein of the classical swine fever virus (CSFV)

The pigtype CSFV Erns Ab is a double-antigen ELISA. The microtiter plate is coated with recombinant CSFV Erns antigen. During sample incubation, antibodies to the CSFV Erns protein bind to the immobilized antigen. Unbound material is removed by rinsing. Antibodies bound to the antigen are detected by a horseradish peroxidase (HRP) conjugate. Unbound conjugate is removed by rinsing. A colorimetric reaction is initiated by adding Substrate Solution and stopped after 10 minutes. If antibodies to the CSFV Erns protein are present in the sample, a blue color develops, which turns yellow after the addition of Stop Solution. The optical density (OD) is measured in a spectrophotometer at 450 nm. Measurement at a reference wavelength (620-650 nm) is optional. The OD value correlates to the concentration of the antibodies to the CSFV Erns protein in the sample.

能檢測(cè)家豬或者野豬的血清和血漿中的豬瘟病毒Erns抗體，配合豬瘟標(biāo)記疫苗（例如碩騰Suvaxyn  CSFV marker等）執(zhí)行豬瘟免疫凈化計(jì)劃，區(qū)分疫苗免疫豬和病毒感染豬。 

豬瘟e0蛋白抗體檢測(cè)試劑盒 豬瘟抗體檢測(cè)檢測(cè) 
酶標(biāo)板上面包被了純化重組豬瘟Erns抗原。樣品經(jīng)過孵育處理后，豬瘟Erns抗體能與包被的抗原特異性結(jié)合。洗板去除沒結(jié)合的物質(zhì)后，每孔加入酶標(biāo)記物孵育。洗板后，將直接使用的底物/顯色劑溶液加入各孔，如果待測(cè)樣品中含有Erns抗體，將發(fā)生藍(lán)色顏色反應(yīng)。孵育完畢，加入終止液終止顏色反應(yīng)并在酶標(biāo)儀450nm測(cè)量。顏色反應(yīng)的顯色強(qiáng)度與樣品中豬瘟Erns抗體含量成正比。