hES-MP 002.5成骨細胞礦化人間充質(zhì)前體細胞是從人胚胎干細胞誘導分化而得到的間充質(zhì)干細胞前體細胞（multipotent mesenchymal progenitors）。
均質(zhì)的，批次檢測的細胞群; 沒有供體到供體的可變問題
細胞強壯易培養(yǎng)
與成人人類間充質(zhì)干細胞高度相似
能夠分化成中胚層譜系
擴增后高礦化潛力

重慶市華雅干細胞技術有限公司，：

華雅集團旗下授權代理TTAKARA Cellartis干細胞產(chǎn)品

圖片為展示效果，實際產(chǎn)品為hES-MP 002.5細胞株

高礦化潛力的間充質(zhì)前體細胞

Cellartis-Y10090 hES-MP 002.5人類胚胎干細胞來源的間充質(zhì)前體細胞

產(chǎn)品英文名稱：hES-MP 002.5，Human Multipotent Mesenchymal Progenitor Cells

產(chǎn)品中文名稱：人類胚胎干細胞來源的間充質(zhì)前體細胞

hES-MP 002.5是從人胚胎干細胞誘導分化而得到的間充質(zhì)干細胞前體細胞（multipotent mesenchymal progenitors）。hES-MP 002.5與人間充質(zhì)干細胞（human mesenchymal stem， MS）高度相似，可以分化成為多種中胚層細胞種類。hES-MP 002.5由人胚胎干細胞系SA002.5 ES（a subclone of SA002）分化得來，可以向骨細胞進行高效率的分化，分化過程具有著高級別的礦物化潛力（mineralization potential）。hES-MP 002.5細胞產(chǎn)品是高度均勻的、高潛力的間充質(zhì)干細胞前體細胞，適合于科研級別以及工業(yè)規(guī)模的間充質(zhì)干細胞生產(chǎn)。

hES-MP 002.5 cells are human embryonic stem (ES) cell-derived multipotent mesenchymal progenitors that resemble adult human mesenchymal stem (MS) cells and can be differentiated into several mesodermal lineages. The cells are derived from the SA002.5 ES cell line, a subclone of SA002, known for efficient osteogenic lineage differentiation and high mineralization potential with extended culture. hES-MP 002.5 cells are a homogeneous, robust source of mesenchymal progenitors, ideal for bulk production of mesenchymal cells.

產(chǎn)品特點

均質(zhì)的，批次檢測的細胞群; 沒有供體到供體的可變問題

細胞強壯易培養(yǎng)

與成人人類間充質(zhì)干細胞高度相似

能夠分化成中胚層譜系

擴增后高礦化潛力High mineralization potential with extended expansion

應用

間充質(zhì)誘導和分化研究

修復和再生的組織工程模型

Mesenchymal induction and differentiation studies

Tissue engineering models of repair and regeneration

2017應用實例：

研究人員使用Cellartis® hES-MP 002.5細胞表明了流動體可以刺激間充質(zhì)干細胞的骨基質(zhì)產(chǎn)生。

A simple rocker‐induced mechanical stimulus upregulates mineralization by human osteoprogenitor cells in fibrous scaffolds

Sasima Puwanun Robin M. Delaine‐Smith Helen E. Colley Julian M. Yates Sheila MacNeil Gwendolen C. Reilly

Journal of Tissue Engineering and Regenerative Medicine,First published: 9 May 2017 https://doi.org/10.1002/term.2462

組份

1瓶細胞

1 frozen vial of Cellartis® hES-MP 002.5

保存：≤ -150°C。

使用方法

Unpacking of Cellartis® hES-MP 002.5

NOTE! For your protection: Wear a protective face mask and protective gloves. Use forceps when

handling a frozen vial. Never hold the vial in your hand as the cryo vial may explode due to rapid

temperature changes.

NOTE! When transferring the cells from the transport vessel to long time storage, immediate transfer is essential since variations in temperature may have an adverse effect on cell survival and quality.

1. Unpack Cellartis® hES-MP 002.5 as soon as possible after arrival.

2. Check all materials for leakage or breakage.

3. Using forceps immediay place the vial in liquid nitrogen at ≤ -150°C.

自備試劑：

產(chǎn)品建議的制造商貨號

basic Fibroblast Growth Factor (bFGF) Peprotech 100-18B

Dulbecco’s Modified Eagle Medium (DMEM) Gibco / Life Technologies 31966-021

胎牛血清（FBS） Gibco / Life Technologies 16140

明膠 Sigma-Aldrich G1890

PBS Dulbecco's w/o Ca2+ & Mg2+ (D-PBS (-/-)) Gibco / Life Technologies 14190

青霉素/鏈霉素（PEST） Gibco / Life Technologies 15140-122

TrypLE Select™ Gibco / Life Technologies 12563-011

產(chǎn)品性能

Cellartis hES-MP 002.5細胞傳30代，細胞群倍加數(shù)（cell population doubling level，縮寫PDL）沒有下降。

Population doubling of human mesenchymal stem cells (hMSCs) and Cellartis hES-MP 002.5 cells through 30 passages. Unlike hMSCs, hES-MP 00.25 cells show no decrease in population doubling level.