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上海希言科學(xué)儀器有限公司>>美國(guó)戴安Dionex>> 085198美國(guó)戴安Dionex MAbPac SCX-10 analytical column 085198

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詳細(xì)介紹

MAbPac SCX-10 Columns

for the High-Resolution Separation and Analysis of Monoclonal Antibody Variants

The Thermo Scientific MAbPac SCX-10 column line has been expanded to include 3 and 5 μm particle sizes for fast, high-resolution analysis as well as rapid separation (RS) columns for bio-UHPLC separations. These smaller-particle-size columns provide the same high resolution as the 10 μm columns, but with significantly faster analysis time. In addition, salt or pH gradients can be used to provide fast high-resolution, high-throughput analysis of monoclonal antibody (MAb) variant samples.

The MAbPac SCX-10 columns are strong cation-exchange columns designed specifically for the high-resolution, high-efficiency analysis of MAbs and associated variants. The unique nonporous pellicular resin provides exceptionally high resolving power, permitting the separation of MAb variants that differ by as little as one charged residue. Hydrophobic interactions with the resin are eliminated for very efficient peaks.

Features:

? Unequalled high-resolution separations
? Exceptionally high efficiency
? Highest resolution of MAb variants
? Unmatched column-to-column and lot-to-lot reproducibility
? Elimination of hydrophobic interactions
? Ideal for stability testing

The MAbPac resin technology is the basis for the superior performance of MAb variant analysis. The nonporous core particle provides high rates of mass transfer, which results in high-efficiency separations. A hydrophilic layer surrounds the polymeric beads, eliminating hydrophobic interactions between proteins and the resin while contributing to high-efficiency peaks. A proprietary grafted cation-exchange surface provides pH selectivity control, resulting in high-resolution separations. In addition, the MAbPac SCX-10 line of columns provides consistent column-to-column and lot-to-lot reproducibility for MAb variant separation results and is ideal for MAb therapeutic method development and high-throughput screening. 

The MAbPac SCX-10 RS columns are designed for use with the Thermo Scientific Dionex  UltiMate 3000 BioRS system. These columns are packed in bioinert, PEEK-lined, stainless steel column hardware suitable for operation up to 10,000 psi. These columns are higher efficiency, and therefore provide higher-resolution separations.

MAbPac SCX-10 columns are designed to address the regulatory requirements for biopharmaceutical characterization. The consistent manufacturing processes ensure reproducibility in methods development and data analysis.

The MAbPac SCX-10 column is complimentary to the industry-leading Thermo Scientific ProPac WCX-10 column for MAb variant analysis. The MAbPac SCX-10 column offers alternative selectivity and provides higher resolution and efficiency for variant analysis of most MAb samples than the ProPac WCX-10 column.

New MAbPac SCX Column Kits for Validation Now Available

  • Three MAbPac SCX-10, 4 × 250 mm columns from one lot of resin
  • Three MAbPac SCX-10, 4 × 250 mm columns, one each from three lots of resin

These MAbPac SCX-10 column kits will facilitate the validation process of MAbPac SCX-10 columns for MAb therapeutics development projects.

The MAbPac SCX-10, 10 μm, 9 × 250 mm column is now available for semipreparative applications of MAb variant separations.

MAbPac SCX-10 Monoclonal Antibody Analysis Column Specifications
Column Dimensions

10 μm: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm, 2 × 250 mm

5 μm Analytical: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm

5 ?m Analytical RS: 4 × 250 mm, 4 × 150 mm

3 μm: 4 × 50 mm

Bead SizeUniform 10 μm, 5 μm, 3 μm particles
SubstrateHighly crosslinked DVB media
Pellicular LayerProprietary hydrophilic
Functional GroupSulfonic
Flow Rate Range:

0.2 to 2 mL/min for: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm

0.1 to 0.5 ml/min for: 2 × 250 mm

Pressure Limit

3,000 psi for 10 μm columns: 4 × 250 mm, 4 × 150 mm, 4 × 50 mm

5,000 psi for Analytical 3 ?m and 5 ?m columns

7,000 psi for Analytical RS 5 ?m columns

Temperature Range:Ambient to 60  ?C
pH Range2–12
Typical buffers:Sodium or potassium salts of phosphate, MES
Solvents:50% acetonitrile if needed for cleaning
Short Term Storage Solution (overnight):Your low salt concentration eluent (pH 3–10)
Shipping Solution / Long Term Storage Solution:20 mM MES (pH 5.6) + 60 mM NaCl + 0.1% sodium azide
Detergent CompatibilityNonionic, anionic, or zwitterionic detergents.  

Analytical Columns
MAbPac SCX-10, 3?m, Analytical Column (4 x 50 mm)077907
MAbPac SCX-10, 5?m, Analytical Column (4 x 50 mm)078656
MAbPac SCX-10, 5?m, Analytical Column (4 x 150 mm)085198
MAbPac SCX-10, 5?m, Analytical Column (4 x 250 mm)078655
MAbPac SCX-10, 10 ?m, Analytical Column (4 x 250mm)074625
MAbPac SCX-10, 10?m, Analytical Column (4 x 150mm)075602
MAbPac SCX-10HT, 10?m, Analytical Column (4 x 50mm)075603
MAbPac SCX-10, 10?m, Analytical Column (2 x 250mm)075604
MAbPac SCX-10, 10?m, 9 x 250 MM columnSP6866
MAbPac SCX-10, 10?m, Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)SP6864
MAPac SCX-10, 10?m, Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)SP6865
Guard Columns
MAbPac SCX-10G, 10?m, Guard Column (4 x 50 mm)074631
MAbPac SCX-10G, 10?m, Guard Column (2 x 50 mm)075749

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