| 概述 |
|
| 描述 | Recombinant Mouse Interleukin-4 is produced by our E.coli expression system and the target gene encoding His23-Ser140 is expressed. |
| 別名 | IL-4蛋白;Interleukin-4;B-cell IgG differentiation factor;B-cell growth factor 1;B-cell stimulatory factor 1;IGG1 induction factor;Lymphocyte stimulatory factor 1;IL-4;BSF-1 |
| 來源 | E.coli |
| 形態(tài) | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, 5% Trehalose, pH 6.5. |
| 性能 |
|
| 純度 | Greater than 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素水平 | Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test. |
| 活性 | Measured in a cell proliferation assay using M-NFS-60 mouse lymphoblast cells. The ED50 for this effect is 0.01 ng/mL. |
| 溶解方法 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 儲存/保存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 靶標 |
|
| 背景說明 | Mouse Interleukin-4(IL-4) is a monomeric, Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four α-helix structure. IL-4 exerts its effects through two receptor complexes, Participates in at least several B-cell activation processes as well as of other cell types. IL-4 is primarily expressed by Th2-biased CD4+T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by na?ve CD4+T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. |
| Accession | Recombinant Mouse IL-4 P07750 |
| 參考文獻 |
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| 參考文獻 | 1、A Novel Rabies Vaccine Expressing CXCL13 Enhances Humoral Immunity by Recruiting both T Follicular Helper and Germinal Center B cells. Wang Z. et al. J Virol. 2017.
2、AID Recognizes Structured DNA for Class Switch Recombination. Qi Qiao. et al. Molecular Cell. 2017. |
| 溫馨提示：本產(chǎn)品僅作科研實驗使用，不支持臨床等研究 |
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