CD43(T細(xì)胞)兔單克隆抗體

廣州健侖生物科技有限公司

CD4主要分布在輔助T細(xì)胞/誘導(dǎo)T細(xì)胞以及吞噬細(xì)胞，正常淋巴組織中，CD4的表達(dá)數(shù)量明顯多于CD8，其比例約為4：1，主要用于皮膚T細(xì)胞淋巴瘤和菌樣霉菌病以及對T細(xì)胞亞群的研究，對后者不能作為區(qū)別腫瘤性或反應(yīng)性T細(xì)胞的參考依據(jù)。

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜

克隆號(hào)：MT1

同型：IgG1

適用組織：石蠟/冰凍

陽性對照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

CD43(T細(xì)胞)兔單克隆抗體

[實(shí)驗(yàn)原理]
血紅蛋白是一種結(jié)合蛋白質(zhì)，由血紅素和珠蛋白組成。正常人所含珠蛋白的多肽鏈有a、b、d 、e、g和z六種。這六種肽鏈的基因在人體發(fā)育的不同階段表達(dá)狀況不一。正常成人紅細(xì)胞中有三種血紅蛋白即HbA、  HbA2 和 HbF。HbA（a2b2）是正常成人紅細(xì)胞中的zui主要的血紅蛋白，占紅細(xì)胞內(nèi)血紅蛋白總量的96%以上，PI=6.7。 HbA2(a2d2) 是正常成人紅細(xì)胞中的次要成分，占2~3% 左右，其PI>6.7。 HbF(a2g2)為胎兒血紅蛋白，胎兒出生后六個(gè)月急劇持續(xù)下降至血紅蛋白總量的1%以下，其PI接近 6.7。各種血紅蛋白在pH8.5的緩沖液中均帶負(fù)電荷在電場中都向陽極移動(dòng)，但因等電點(diǎn)不同所帶電荷數(shù)目不同而有不同的電泳速度。HbA因其所帶電荷zui多故向陽極泳動(dòng)速度zui快，又因其含量zui多區(qū)帶顏色zui深。其后有一較淺的區(qū)帶為HbA2，HbF與HbA的等電點(diǎn)接近，通常與HbA分不開。
異常血紅蛋白是指由于基因突變而導(dǎo)致結(jié)構(gòu)異常的紅蛋白。異血紅蛋白具有與正常血紅蛋白不同的電泳行為，因此用電泳的方法可以將其檢出。例如HbS是一種由b-鏈第6位的谷氨酸被纈氨酸所替換而形成的異常血紅蛋白，此變異體比HbA少帶兩個(gè)負(fù)電荷，因此，向陽極移動(dòng)的速度比HbA慢，出現(xiàn)于HbA與HbA2之間。
分析血紅蛋白的電泳方法有許多，如濾紙電泳，淀粉膠電泳及聚丙烯酰胺凝膠電泳等。但異常血紅蛋白篩查zui常用的還是醋酸纖維薄膜電泳法。因?yàn)榇姿崂w維薄膜做支持體電泳操作簡便，電泳速度快，分辨力強(qiáng)區(qū)帶集中而且對染料不吸附，容易定量。用醋酸纖維素薄膜電泳篩查異常血紅蛋白的檢出率為1~2‰。

CD43(T細(xì)胞)兔單克隆抗體

我司還提供其它進(jìn)口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

[Experimental principle]
Hemoglobin is a binding protein that consists of heme and globin. Normal human globin chain containing a, b, d, e, g and z six kinds. The genes for these six peptide chains are expressed differently at different stages of human development. There are three types of hemoglobin in normal adult erythrocytes, HbA, HbA2 and HbF. HbA (a2b2) is the most common hemoglobin in normal adult erythrocytes, accounting for more than 96% of the total hemoglobin in erythrocytes, PI = 6.7. HbA2 (a2d2) is a secondary component of normal adult red blood cells, accounting for about 2 to 3%, the PI> 6.7. HbF (a2g2) is fetal hemoglobin, and the fetus continues its sharp decline to less than 1% of total hemoglobin six months after birth with a PI of approximay 6.7. Various hemoglobin in the pH8.5 buffer are negatively charged in the electric field are moving toward the anode, but due to different isoelectric point with different charge numbers and have different electrophoresis velocity. HbA has the fastest charge to the anode and has the deepest color because of its highest content. Subsequent to a lighter zone HbA2, HbF and HbA isoelectric point close, usually inseparable from the HbA.
Abnormal hemoglobin refers to structural abnormalities due to genetic mutations in the hemoglobin. Hemoglobin has different electrophoretic behavior than normal hemoglobin, so it can be detected by electrophoresis. For example, HbS is an abnormal hemoglobin formed by the substitution of glutamic acid at position 6 of the b-chain by valine. This variant has two negative charges with respect to HbA and therefore moves toward the anode more slowly than HbA , Appeared between HbA and HbA2.
Analysis of hemoglobin electrophoresis methods are many, such as filter paper electrophoresis, starch gel electrophoresis and polyacrylamide gel electrophoresis. However, abnormal hemoglobin screening is the most commonly used acetate film electrophoresis. Because acetate film support electrophoresis operation is simple, fast electrophoresis, strong resolution zone with dye and non-adsorption, easy to quantify. The detection rate of abnormal hemoglobin by cellulose acetate membrane electrophoresis was 1 ~ 2 ‰.