登革熱NS1抗原檢測試紙

 產(chǎn)品規(guī)格：25T/盒； 

 保存條件：避光 4-30度 保存。

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登革熱NS1抗原檢測試紙

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登革熱NS1抗原檢測試紙
 

9.      Pipette 100 µl streptavidin-HRP (ELISA kits) or anti-rabbit IgG-HRP (phosphoELISA™ kits) solution to each well except the chromogen blank(s).
10.  Cover wells with the plate cover and incubate for 30 minutes at room temperature.
11.  Thoroughly aspirate or decant solution from wells and discard the liquid. Wash wells 4 times.
12.  Pipette 100 µl of Stabilized Chromogen to each well. The liquid in the wells will begin to turn blue.
13.  Incubate for 30 minutes at room temperature and in the dark. Note: Do not cover the plate with aluminum foil or metalized mylar. The incubation time for chromogen substrate is often determined by the microtiter plate reader used. Many plate readers have the capacity to record a maximum optical density (OD) of 3.0. The OD values should be monitored and the substrate reaction stopped before the OD of the positive wells exceed the limits of the instrument. The OD values at 450 nm can only be read after the Stop Solution has been added to each well. If using a reader that records only to 3.0 OD, stopping the assay after 20 to 25 minutes is suggested.
14.  Pipette 100 µl of Stop Solution to each well. Tap gently on the side of the plate to mix. The solution in the wells should change from blue to yellow.

9。管啊100µL親和齋（ELISA試劑盒）或者抗兔IgG抗體（phosphoelisa™套件）解決方案呀個(gè)個(gè)除咗咸濕空白（S）。
10。起與起威爾斯，哺育半個(gè)鐘喺室溫。
11。*吸出或者倒瀉溶液由威爾斯同廢液。洗威爾斯4次。
12。管啊100µL穩(wěn)定發(fā)色團(tuán)喺每。威爾斯體內(nèi)嘅液體開始變藍(lán)。
13。哺育半個(gè)鐘，喺室溫下，喺鼆中。注：唔包板用鋁箔或者金屬化聚酯薄膜。對(duì)咸濕底物哺育時(shí)間往往系由讀者用微孔板。好多平板閱讀器能夠紀(jì)錄3嘅zui大光密度（OD）。喺正向威爾斯嘅OD值超過儀器嘅極限前，應(yīng)監(jiān)測OD值同底物反應(yīng)閘住。只有喺每井加閘住液后先可以讀取450 nm處嘅OD值。如果使用只紀(jì)錄到3 OD嘅讀取器，建議喺二十到25分鐘后閘住檢測。
14。管啊100µl閘住解決每。細(xì)仔噉喺碟嘅一路細(xì)仔噉攪拌。威爾斯中嘅溶液應(yīng)該由藍(lán)變?yōu)辄S色。