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3D單分子熒光成像系統(tǒng)-SAFe 360簡介：
SAFe 360是法國abbelight公司推出的款基于單分子定位的顯微成像（SMLM）的新型3D單分子成像系統(tǒng)，它*的DAISY技術(shù)整合了散光技術(shù)和超臨界角光技術(shù)，能夠很大的提高定位精度，xyz三軸定位精度高達(dá)15nm，可以提供高清晰三維亞細(xì)胞結(jié)構(gòu)圖像，支持同時多四色成像，可以用于細(xì)胞納米三維成像，觀測高清晰亞細(xì)胞器結(jié)構(gòu)，實(shí)時研究不同的結(jié)構(gòu)功能蛋白的共定位信息，在單分子水平研究分子動力學(xué)反應(yīng)以及細(xì)胞間的相互作用等。
| 加裝 | TIRF smFRET ...... | 兼容 | Confocal STED ...... |
3D單分子熒光成像系統(tǒng)-SAFe 360設(shè)備參數(shù)
+ 成像模式：PALM、STORM、PAINT、smFRET 、SPT
+ 光源模式：Epi、TIRF、HILO
+ 分辨率：15 nm的XYZ軸分辨率
+ 超大視野：200 × 200 μm2的視野
+ 次可同時采集1.2 μm深度圖像信息
+ 圖像深度：10 μm
+ 實(shí)時漂移矯正
+ 四色同時成像
+ 活細(xì)胞成像模式
配套試劑
Smart kit • 10 doses per box • 200 µL per dose • 30 sec prepartion • 2 months in a fridge • 2 weeks on sample | Compatible dyes • Atto 488, WGA-AF®488 • AF®532, CF®532, Cy3b • AF®555, AF®594, CF®555, AF®568, CF®568, Cy5, MemBriteTM 568, TMR • AF®647, CF®647, AF®680, CF®680, MemBriteTM 640, Actin-stain 670, SiR647 |
測試數(shù)據(jù)
3D線粒體結(jié)構(gòu) |
核孔復(fù)合物 |
老鼠海馬神經(jīng)元 |
微管蛋白網(wǎng)絡(luò) |
發(fā)表文章
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[13] Culley, Sian, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods 15.4 (2018): 263.
[14] Berger, Stephen L., et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron 97.3 (2018): 555-570.
[15] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters 43.2 (2018): 174-177.
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用戶單位
