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93341ESRecombinant Human CD3E&CD3D/CD3 epsilon &
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產(chǎn)品簡(jiǎn)介
| 供貨周期 | 兩周 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè),制藥 |
|---|
產(chǎn)品介紹
產(chǎn)品信息
產(chǎn)品名稱 | 產(chǎn)品編號(hào) | 規(guī)格 |
Recombinant Human CD3E&CD3D/CD3 epsilon & CD3 delta (His Tag) | 93341ES60 | 100 μg |
93341ES76 | 500 μg | |
93341ES80 | 1 mg |
產(chǎn)品描述
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
產(chǎn)品性質(zhì)
別名（Synonyms） | CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
Uniprot No. | P07766(CD3E)&P04234(CD3D) |
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） | Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3D). |
分子量(Molecular Weight) | The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result. |
外觀(Physical Appearance） | Sterile Filtered White lyophilized (freeze-dried) powder. |
純度（Purity） | > 95% as determined by SDS-PAGE and HPLC |
活性（Activity） | 1.ELISA Data：Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA. 2.ELISA Data：Immobilized Human CD3E&CD3D at 2ug/ml (100μl/Well). Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. |
內(nèi)毒素（Endotoxin） | < 1.0 EU per 1μg of the protein by the LAL method. |
制劑（Formulation） | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
復(fù)溶（Reconstitution） | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃至-80℃保存，一年有效期。
復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后，2-8°C 保存 2-7 天。
建議第一次使用時(shí)分裝凍存，避免反復(fù)凍融。
注意事項(xiàng)
1.避免反復(fù)凍融。
2.為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
3.本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
Tris-Bis PAGE
Human CD3E&CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
SEC-HPLC
The purity of Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.
ELISA Data
Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA.
ELISA Data
Immobilized Human CD3E&CD3D at 2ug/ml (100μl/Well).
Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA.
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