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14615ESRNase R GMP-grade (20 U/μL)
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- 曹女士
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- 400-6111-883
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產(chǎn)品簡介
| 供貨周期 | 現(xiàn)貨 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè) |
|---|
Ribonuclease R (RNase R) is a magnesium-dependent 3'→5' exoribonuclease that can digest essentially all linear RNAs, but does not digest lariat or circular RNA structures, or doubl
產(chǎn)品介紹
Product Description
Ribonuclease R (RNase R) is a magnesium-dependent 3'→5' exoribonuclease that can digest essentially all linear RNAs, but does not digest lariat or circular RNA structures, or double-stranded RNA with 3' overhangs shorter than 7 nucleotides. As such, this enzyme is ideally suited to the study of lariat RNA produced by traditional splicing, as well as circRNAs which arise through back-splicing. By removing linear RNAs from cellular or RNA extracts, RNase R greatly facilitates the identification of circular species through RNA-sequencing. This enables researchers to probe the landscape of splicing events with greater depth.
This product is produced in accordance with GMP process requirements and provided in liquid form.
Specifications
Expression Host | Recombinant E. coli with RNase R gene |
Storage Buffer | 50 mM Tris-HCl, 100 mM NaCl, 0.1 mM EDTA, 1mM DTT, 0.1% TritonX-100, 50% Glycerol, pH 7.5 |
Reaction Temperature | 37℃ |
Unit Definition | One unit converts 1 μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37 ℃. |
Application | 1.Identification of intronic lariat sequences 2.Identification of exonic circRNAs 3.Studying alternative splicing 4.Production of artificial circular RNAs |
Components
Components No. | Name | 14615ES25 (500 U) | 14615ES72 (5000 U) | 14615ES80 (20 KU) | 14615ES92 (200 KU) |
14615 | RNase R (20 U/μL) | 25 μL | 250 μL | 1 mL | 10 mL |
Shipping and Storage
This product is shipped with dry ice and can be stored at -25 ~ -15℃ for two years.
Instructions
Experimental methods
The following reaction system was formulated in a sterile micro-centrifuge tube:
Components | Volume |
10 × RNase R Reaction Buffer* | 2 μL |
RNA Sample | 1 μg |
RNase R (20 U/μL) | 2-4 U |
DEPC H2O | Up to 20 μL |
*According to the specific purpose of experiments, it is necessary to prepare the corresponding reaction buffer. You can consider buying 14616ES(10×RNase R Reaction Buffer GMP-grade) to use together.
Reaction condition：37℃ for 10 min to 30 min.
Inactivation condition: incubation at 70℃ for 10 min can inactivate the enzyme.
Note
For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product
HB241203
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